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在Tribolium castaneum嵌入式和输入式Paralogs的功能分歧

Summer Blunk¹, Hector Garcia-Verdugo¹, Sierra O'Sullivan¹

¹Molecular and Cellular Biology, University of Arizona, Tucson, AZ 85721, USA.

|August 25, 2023

概括

嵌入式 (en) 和引入式 (inv) 基因对于昆虫细分的发展至关重要. 它们在Tribolium的联合淘汰导致严重的细分缺陷,揭示了昆虫系之间的协同作用和独立的功能进化.

科学领域:

发展生物学发展生物学
遗传学是一个遗传学.
进化生物学进化生物学

背景情况:

嵌入式 (en) 和嵌入式 (inv) 是整体代谢昆虫中类似的转录因子,来自于协同重复.
虽然Drosophila en 突变不能维持细分,而 inv 突变是可行的,但组合损失会导致细分幼虫.
之前的研究表明,Oncopeltus和Tribolium分别在inv或en敲击时出现了各种细分缺陷,但除了Drosophila之外,双相对应损失效应仍然未被研究.

研究的目的:

在Tribolium胚胎生成中研究en和inv对应物的协同功能.
阐明了En/Inv介导的细分损失背后的分子机制.
通过对比昆虫谱系中en和inv的功能进化.

主要方法:

的模型生物.Tribolium castaneum模型生物.
RNA干扰 (RNAi) 对于单独或组合的en和inv的基因淘汰.
皮肤质缺陷和尾发育的表型分析.
在en/inv knockdown胚胎中对inv和 hedgehog (hh) 的基因表达分析.

主要成果:

对Tribolium en和inv的双重打击导致了严重的表型:小的,无肢的,背部开放的幼虫,只有一排毛发.
不那么严重的双重击倒表现出融合的细分和减少的附属物.
单一的en或inv的淘汰通常不那么严重,极端情况下模仿双重淘汰表型,表明协同作用.
对于单基因淘汰,没有观察到独特的形态异常.
inv表达不受en knockdown的影响,但 hedgehog (hh) 表达在开发过程中增加.

结论:

三角形en和inv对应物协同工作,以确保适当的细分发展.
虽然昆虫的en/inv细分表达被保留,但它们的特定功能正在独立进化.
这项研究突出了不同昆虫系中相似基因的功能演变的差异.

关键词:

在Tribolium上,我们可以看到三角.嵌入式嵌入式基因对应物基因对应物他们被投诉了,被投诉了.细分极性 - 细分极性顺序细分的分段化顺序的细分化

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