在二次代谢物Trichoderma atroviride的产生中,光引起的变化
Kristina Missbach1,2, Daniel Flatschacher2, Christoph Bueschl1
1Department of Agrobiotechnology IFA-Tulln, Institute of Bioanalytics and Agro-Metabolomics, University of Natural Resources and Life Sciences Vienna (BOKU), 3430 Tulln, Austria.
Journal of fungi (Basel, Switzerland)
|August 25, 2023
概括
在减少光线 (RL) 中培养Trichoderma atroviride可以最大限度地提高真菌的二次代谢物产量. 恒定的黑暗 (PD) 或RL产生了类似的结果,而光/黑暗周期抑制了代谢物形成.
科学领域:
- 菌类学 菌类学是指菌类学.
- 代谢学 代谢学 代谢学
- 生物技术是生物技术.
背景情况:
- 菌的二次代谢物对各种应用至关重要.
- 优化真菌种植条件是最大限度地提高代谢产量的关键.
- 光线对真菌二次新陈代谢的影响经常被忽视.
研究的目的:
- 系统地研究不同照明模式对Trichoderma atroviride中二次代谢物产生的影响.
- 为了比较不断黑暗 (PD),减少光线 (RL) 和12/12小时的光/黑暗周期 (LD) 下的代谢物概况.
主要方法:
- 基于非向的同位素辅助液态染色学高分辨率质谱学 (LC-HRMS) 的代谢学.
- 在三种明确的光照条件下标准化培养Trichoderma atroviride.
- 代谢物概况和空间分布的比较分析.
主要成果:
- 减少光线 (RL) 和持续黑暗 (PD) 的种植导致了类似的代谢物概况.
- 一个12/12小时的光/黑暗周期 (LD) 显著抑制了二次代谢产品的数量和丰度.
- 在500多种检测到的代谢物中,只有很小一部分是完全在黑暗中产生的,或者在黑暗中更加丰富.
结论:
- 在减少光线下 (RL) 种植是一种可行的策略,以最大限度地提高Trichoderma atroviride的二次代谢产量.
- 光,特别是循环光,可以作为这种真菌中二次新陈代谢的抑制因素.
- 了解光线的影响为优化真菌生物过程提供了新的途径.
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