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生物反应器Spodoptera frugiperda多核聚合体病毒生物杀虫剂的生产过程

Karina Klafke¹, Marcio Martinello Sanches², William Sihler³

¹Department of Chemical Engineering, Polytechnic School, University of Sao Paulo, Sao Paulo 05508-010, SP, Brazil.

Pathogens (Basel, Switzerland)

|August 25, 2023

概括

在实验室中大规模生产Spodoptera frugiperda多重核聚合体病毒 (SfMNPV) 使用生物反应器进行了优化. 在补充培养基中,感染倍数为1.0时,获得了最佳的病毒生成.

科学领域:

病毒学病毒学
昆虫病理学昆虫病理学
生物技术是生物技术.

背景情况:

虫 (Spodoptera frugiperda) 是一个主要的全球玉米害虫.
斯波多 (Spodoptera frugiperda) 多重核多重体病毒 (SfMNPV) 是一种作为生物杀虫剂使用的天然病原体.
目前的商业生产依赖于体内方法,面临大规模生产的局限性.

研究的目的:

开发和优化SfMNPV的体外生产过程.
评估多重感染 (MOI) 和营养补充剂对SfMNPV生产的影响.
分析SfMNPV感染对Sf9细胞生长和病毒产量的影响.

主要方法:

使用动反应器 (STR) 生物反应器系统用于SfMNPV种植.
使用的Sf-900 III无血清培养基补充了谷氨.
测试了各种多重的感染 (MOI) 并分析了细胞生长,形态和分子标记.

主要成果:

最佳条件涉及的感染多重性 (MOI) 为1.0.
在Sf-900 III介质中补充谷氨胺可以增强病毒的产生.
实现了超过300个封闭体 (OBs) /细胞的特定生产率和9.0 × 10 ^ 11 OBs / L. 的体积生产率.

结论:

动反应堆 (STR) 方法与特定的MOI和中等补充是有效的大规模SfMNPV体外生产.
这种优化的 in vitro 方法为传统的 in vivo baculovirus 大规模生产提供了可行的替代方案.
这些发现有助于使用生物杀虫剂的可持续害虫管理策略.

关键词:

这是SfMNPVV.它们是花鸟 (Spodoptera frugiperda) 的种类.细菌病毒的病毒.生物控制生物控制生物反应器是一个生物反应器.秋天的军队虫在体外生产 in vitro生产.

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