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在尼泊尔加德满都山谷的牛群中分子检测和识别热质体

Medhavi Dhakal¹, Tulsi Ram Gompo², Prakash Devkota²

¹Central Department of Zoology, Institute of Science and Technology, Tribhuvan University, Kathmandu 44601, Nepal.

Pathogens (Basel, Switzerland)

|August 25, 2023

概括

分子方法在尼泊尔牛中发现了传播原生虫寄生虫 (TBPPs),包括Babesia和Theileria物种. 这项研究为开发该地区有效的TBPP监测和预防策略提供了关键数据.

科学领域:

兽医寄生虫学兽医寄生虫学
分子诊断学分子诊断学
原生动物学原生动物学.

背景情况:

传播的原生虫寄生虫 (TBPPs) 对尼泊尔的家禽健康构成重大威胁.
目前的诊断方法依赖于劳动密集型显微镜,在牛中对Babesia和Theileria的物种鉴定有限.
需要先进的分子技术来准确地识别尼泊尔牛群中的TBPP.

研究的目的:

通过分子方法识别感染尼泊尔牛的传播原生类寄生虫物种.
为了比较嵌套PCR (nPCR) 和序列分析与传统显微镜用于TBPP检测的疗效.
为该地区未来的TBPP监测和控制计划建立基准.

主要方法:

从加德满都谷收集了106只牛的血液样本.
用显微镜检查薄血涂片,初步检测寄生虫.
DNA提取,然后进行常规嵌套PCR (nPCR) 和用于物种识别的直接测序.

主要成果:

嵌套PCR在52.8%的样本中检测到皮洛寄生虫,超过了显微镜检测率 (42.5%).
已识别的物种包括Babesia bigemina,Babesia bovis,Theileria annulate和Theileria orientalis.这些物种都被发现了.
遗传学分析证实了B. bovis,B. bigemina和T. orientalis的不同的分类,T. annulate显示了两个不同的分类.
与健康动物相比,受皮洛菌感染的牛的血红蛋白和红细胞数量减少.

结论:

这项研究代表了尼泊尔首次对牛中的TBPPs进行分子检测和物种确定.
已识别的TBPP物种及其遗传多样性为有针对性的干预提供了必要的数据.
这些发现为在尼泊尔制定有效的TBPP监测和预防战略奠定了基础.

关键词:

贝贝西亚贝贝西亚是一个尼泊尔尼泊尔尼泊尔尼泊尔尼泊尔这是一个PCRPCR.泰勒里亚是他们的家园.这里是牛群.

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