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Updated: Jul 18, 2025

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一种可遗传编码的化光环交联剂,通过光诱导的脱化酸交换

Jielin Fu1, Sitong Li1, Lijun Deng1

  • 1Key Laboratory of Green Chemistry and Technology of Ministry of Education, College of Chemistry, Sichuan University, 29 Wangjiang Road, Chengdu 610064, P. R. China. zhipengy@scu.edu.cn.

Chemical communications (Cambridge, England)
|August 25, 2023
PubMed
概括

研究人员开发了一种新的氨基酸,m-trifluoromethylaniline修饰L-lysine (m-TFMAK),可以被遗传编码. 在光激活时,m-TFMAK可实现精确的蛋白质交联,用于研究生物系统内的相互作用.

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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