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Zer Vue¹, Kit Neikirk¹, Larry Vang¹

¹Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, Tennessee, United States.

American journal of physiology. Heart and circulatory physiology

|August 25, 2023

概括

衰老会损害心肌中的线粒体,导致晶状体结构的损失和体积的减少. 这项研究调查了MICOS复合体在这些与年龄有关的心脏线粒体变化中的作用.

科学领域:

心血管生物学心血管生物学
线粒体生物学线粒体生物学
衰老研究研究衰老研究

背景情况:

心脏病是导致死亡的主要原因,由于线粒体功能障碍,衰老会损害心脏效率.
与线粒体相关的过程,如氧化应激和生物能学,都与心力衰竭有关.
线粒体接触点和组织系统 (MICOS) 在心脏衰老中的作用基本上是未知的.

研究的目的:

为了研究心脏线粒体中与年龄相关的结构变化.
为了确定MICOS复合体在衰老期间心脏线粒体变化中的参与.

主要方法:

使用传输电子显微镜 (TEM) 和串行块面扫描电子显微镜 (SBF-SEM) 对心肌线粒体3D网络的定量分析.
对3个月,1岁和2岁的雄性小鼠的分析.
与具有CRISPR/Cas9敲除MICOS复合组件 (Mitofilin,Chchd3,Chchd6) 和Opa1.6的小鼠心脏纤维细胞进行比较.

主要成果:

随着年龄的增长观察到线粒体晶体形态的显著损失.
在老年小鼠中观察到心脏线粒体3D体积的减少.
纤维细胞中MICOS成分和Opa1的遗传淘汰模仿了与年龄相关的线粒体表型,包括减少氧化消耗和线粒体体积.

结论:

与年龄相关的心脏线粒体结构变化包括晶状体的丧失和线粒体体积的减少.
在衰老的心肌中,MICOS复合体可能在维护线粒体超结构方面发挥关键作用.
需要进一步的研究来探索MICOS复合体丧失在与年龄有关的心脏线粒体功能障碍中的具体参与.

关键词:

麦科斯 (MICOS) 是一个小岛屿.衰老的衰老衰老的衰老心肌线粒体心肌线粒体串行块面SEMM可以使用.三维形态测量三维形态测量

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