储存温度和时间对藻类度的影响
Jensen E Cherewyk1, Taylor J Grusie-Ogilvie2, Sarah E Parker3
1Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK S7N 5B4, Canada.
Toxins
|August 25, 2023
概括
储存条件显著影响受污染的谷物中的藻类水平. 藻类度随着时间的推移而变化,R-epimer不如S-epimer稳定,无论温度如何.
科学领域:
- 农业科学 农业科学
- 分析化学 分析化学
- 食品安全 食品安全
背景情况:
- 杂草化物是杂草硬化菌感染谷物作物和草的有毒代谢物.
- 这些类存在于两个异构体:C-8-R-异构体 (R-epimer) 和C-8-S-异构体 (S-epimer).
- 像谷物一样受污染的矩阵通常在分析或消费之前长期存储.
研究的目的:
- 为了评估自然污染的小麦中六种常见的藻类的稳定性,随着时间的推移.
- 为了确定不同储存温度 (室温,+4°C,-20°C) 对藻类度的影响.
- 为了评估储存下的R-epimer和S-epimer配置的稳定性.
主要方法:
- 自然污染的小麦样本存储了一个,两个和四个月.
- 样品在室温,+4°C和-20°C下储存.
- 使用超高性能液体染色学-并联质谱法 (UHPLC-MS/MS) 量化了草类度.
主要成果:
- 随着时间的推移和温度的变化,总藻类度发生了变化.
- 度在两个月后达到峰值,并在四个月后下降,无论温度如何 (p < 0.05).
- 与S-epimer相比,R-epimer度在时间上的稳定性较低.
结论:
- 储存时间和温度是影响受污染的基质度的关键因素.
- 在使用前应进行藻类化物的量化,以便进行准确的评估.
- 可能与ergocristine/ergocristinine相关的R和S epimers的差异稳定性需要在风险评估中考虑.
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