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对猪和鹿中Mycobacterium bovis感染的抗体测试的评估

Penny Barton¹, Nick Robinson¹, Sonya Middleton¹

¹Animal and Plant Health Agency (APHA), Weybridge KT15 3NB, UK.

Veterinary sciences

|August 25, 2023

概括

在鹿和猪的四个牛结核病 (TB) 抗体测试显示高特异性. 在皮肤测试后,鹿的灵敏度增加了25%,这有助于结核病控制策略.

科学领域:

兽医免疫学兽医免疫学
传染病诊断传染病诊断传染病诊断
野生动物健康野生动物健康

背景情况:

鹿和猪的牛结核病 (TB) 对英国的动物健康构成重大挑战,需要可靠的诊断工具.
对被证实感染了Mycobacterium bovis的群体实施了移动限制,需要对群体退出策略进行有效的测试.
现有的结核病检测方法可能需要补充方法来提高灵敏度和可靠性.

研究的目的:

评估四项补充抗体测试的性能,以检测鹿和猪中的Mycobacterium bovis感染.
评估结核素皮肤测试对鹿类抗体测试敏感性的影响 (历史记录增强效应).
为结核病受限制群体提供支持开发退出策略的数据.

主要方法:

从确定的结核病感染和没有结核病的鹿和猪队伍中收集了血清样本.
评估了四种抗体测试:DPP VetTB Assay for Cervids,IDEXX ELISA,Enferplex Cervid和猪,以及一个内部的PPD ELISA.
鹿队包括最近进行皮肤测试和没有进行皮肤测试的养殖鹿,猪包括养殖动物和野猪.

主要成果:

所有四个抗体测试都显示出对鹿和猪的高特异性 (98.8-99.5%).
皮肤测试增强的感染鹿的敏感度在76.6-85.7%之间,与未增强的鹿相比显著增加 (51.4-58.1%).
猪抗体测试显示高特异性 (99-99.5%) 和可变敏感性 (62.1-86.2%),三种测试之间有很好的一致性. 追溯分析检测到野猪中低水平的M. bovis.

结论:

评估的抗体测试为鹿和猪的结核病检测提供了高特异性.
结核素皮肤测试增强显著提高了感染鹿的抗体测试灵敏度,这表明了协同的诊断方法.
这些抗体测试提供了可靠的评估,这对于在结核病控制计划中更广泛地部署这些抗体的信心至关重要.

关键词:

DPP VetTBTB 在线观看艾丽莎·伊丽莎·伊丽莎这是一个Enferplex.IDEXXXX 是一个名字.这种细菌是Mycobacterium bovis.抗体是一种抗体.鹿鹿是什么意思鹿是什么意思诊断诊断诊断的诊断诊断诊断诊断的诊断侧向流动的侧向流动猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪猪

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