Sarcocystis 在立陶宛的绵羊和山羊中的物种丰富性
Alina Marandykina-Prakienė1, Dalius Butkauskas1, Naglis Gudiškis1
1Nature Research Centre, Akademijos 2, 08412 Vilnius, Lithuania.
Veterinary sciences
|August 25, 2023
概括
分子分析揭示了羊和山羊中意想不到的Sarcocystis物种,挑战了已有的宿主特异性. 这项研究在这些农场动物中确定了非正规的寄生虫,需要进一步研究.
科学领域:
- 兽医寄生虫学 兽医寄生虫学
- 分子生物学分子生物学
- 食品 动物健康 动物健康
背景情况:
- 农场动物中Sarcocystis物种的宿主特异性尚未完全理解.
- 之前的研究显示,关于Sarcocystis spp.中间宿主特异性的数据是矛盾的.
研究的目的:
- 在立陶宛的绵羊和山羊中分子识别Sarcocystis物种.
- 在这些小反动物中调查可疑的非正规寄生虫.
- 为了澄清Sarcocystis spp.的宿主特异性.
主要方法:
- 收集了47只绵羊和9只山羊的肌肉样本.
- 使用针对cox1基因的直接和嵌套PCR识别sarcocystis物种.
- 积极放大PCR产品的测序.
主要成果:
- 检测到正规的萨尔科囊 spp. 在他们预期的中间宿主中.
- 在羊群中鉴定了Sarcocystis capracanis和Sarcocystis morae的DNA.
- 在山羊中发现了sarcocystis arieticanis和sarcocystis tenella的DNA.
- 光显微镜只证实了山羊中的Sarcocystis capracanis囊.
结论:
- 这项研究在绵羊和山羊中确定了与这些宿主通常不相关的Sarcocystis物种.
- 这些发现挑战了Sarcocystis capracanis,Sarcocystis morae,Sarcocystis arieticanis和Sarcocystis tenella所谓的宿主特异性.
- 建议进一步研究这些Sarcocystis物种的生命周期和宿主特异性.
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