相关实验视频

Updated: Jul 18, 2025

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

A Protocol to Characterize the Morphological Changes of Clostridium difficile in Response to Antibiotic Treatment

Published on: May 25, 2017

来自癌症患者的分子特征和基因型鉴定,这些患者患有 Clostridioides difficile 感染或无症状殖民

Daniel de-la-Rosa-Martinez^1,2, Miriam Bobadilla Del Valle³, Veronica Esteban-Kenel³

¹Plan de Estudios Combinados en Medicina (PECEM), Faculty of Medicine, Universidad Nacional Autonoma de Mexico, México City, Mexico.

Journal of medical microbiology

|August 25, 2023

概括

在癌症患者中,Clostridioides difficile无症状殖民 (CDAC) 与C. difficile感染 (CDI) 具有克隆性联系. 了解CDAC分子特征是控制CDI在易受癌症影响的人群中传播的关键.

科学领域:

微生物学微生物学
传染性疾病传染性疾病
在瘤学瘤学.

背景情况:

癌症患者面临着Clostridioides difficile感染 (CDI) 的风险增加和C. difficile无症状殖民 (CDAC) 的高率.
癌症患者中CDAC的分子特征和传播作用仍未得到充分研究.

研究的目的:

为了比较来自癌症患者的C. difficile分离物的分子和基因型特征,CDAC和CDI.
调查CDAC在癌症患者中与医疗保健相关的传播中的潜在作用.

主要方法:

对患有CDAC或CDI的癌症患者进行前性队列研究.
对C. difficile分离物的分子特征,菌株类型和tcdC基因表达分析.
对毒素基因存在 (tcdA,tcdB,ctdA,ctdB) 和tcdC突变的分析.

主要成果:

分析了37个CDI和14个CDAC分离物. 所有的CDI分离物都具有毒性 (tcdA+/tcdB+).
71%的CDAC分离物具有毒性. 有关毒性CDAC分离物的很大一部分 (60%) 与CDI分离物具有克隆相关性.
特定的tcdC缺失和突变在一组分离物中被确定.

结论:

在癌症患者中,CDAC和CDI的C. difficile分离物之间存在克隆关联.
需要进一步的研究来阐明无症状携带者在非住院性CDI传播中的作用.
这些发现可能会为感染控制策略提供信息,以减少高风险癌症患者群体的CDI负担.

关键词:

这是C. difficile.克洛斯特里迪奥伊德困难感染.无症状的携带者是无症状的没有症状的殖民化.没有症状的感染.癌症癌症癌症癌症癌症

更多相关视频

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

A Protein Microarray Assay for Serological Determination of Antigen-specific Antibody Responses Following Clostridium difficile Infection

Published on: June 15, 2018

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Cefoperazone-treated Mouse Model of Clinically-relevant Clostridium difficile Strain R20291

Published on: December 10, 2016

相关概念视频

Modern Molecular Taxonomy

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...

Applications of Molecular Taxonomy

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...