微RNA-155促进体中的功能突变调节其转录效率和表达
Congcong Li1, Wanxia Zhao2, Huijie Zhou2
1College of Animal Science and Technology, Henan University of Animal Husbandry and Economy, No. 6 Longzi North Road, Zhengdong New District, Zhengzhou, 450046, Henan, China. congcong_925520@126.com.
Molecular biotechnology
|August 25, 2023
概括
调查猪miR-155促进体发现了两个关键的转录因子,Foxp3和RelA. 特定的突变部位显著影响microRNA-155表达,为猪提供潜在的疾病耐药性标志物.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 动物科学动物科学
背景情况:
- 关于猪微RNA-155 (miR-155) 促进体的研究有限.
- 之前的研究已经确定了两种与miR-155基因中突变部位相关的哈普洛类型 (TT和CC).
研究的目的:
- 为了选猪miR-155促进器区域.
- 为了确定调节miR-155.5的转录因子.
- 为了研究特定突变部位对miR-155表达的影响.
主要方法:
- 促进体查和转录因子 (Foxp3,RelA) 的鉴定.
- 电泳运动转移试验 (EMSA) 评估蛋白质-DNA结合.
- 在体外表达分析和北方涂抹以量化miR-155水平.
主要成果:
- 福克斯p3结合受第一个突变部位 (A/C) 的影响.
- 而C型单体表现出显著更高的miR-155表达比T型单体表现.
- 第一个 (A/C) 和第四个 (G/T) 突变部位都显著影响了miR-155的表达.
结论:
- 确定了Foxp3和RelA作为猪miR-155转录的关键调节者.
- 确定了影响miR-155表达水平的关键突变部位.
- 建立了开发猪病耐药性的分子标记物的基础.
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