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Updated: Jul 18, 2025

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

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Published on: January 31, 2025

TMED10调解了新合成的Sonic Hedgehog在COPII膀中的加载,以有效分泌和信号传递

Yonis Bare^1,2, Tamás Matusek³, Sophie Vriz^4,5

¹Institut de Recherche en Infectiologie de Montpellier (IRIM) CNRS, 1919 Route de Mende, 34293, Montpellier, France.

Cellular and molecular life sciences : CMLS

|August 25, 2023

概括

新合成的Sonic Hedgehog (SHH) 使用TMED10从内质网膜传输到Golgi,然后传输到细胞表面,揭示了对胚胎发育信号的新见解.

科学领域:

细胞生物学细胞生物学
发展生物学发展生物学
分子机制的分子机制

背景情况:

索尼克 (SHH) 是胚胎发育的关键形态原体.
SHH信号涉及多种分子形式和复杂的贩运途径.
哺乳动物细胞中新合成的SHH的早期贩运机制尚不清楚.

研究的目的:

阐明控制新合成SHH早期贩运的分子机制.
通过分泌途径识别参与SHH运输的关键蛋白质.
调查SHH贩运成分在胚胎发育中的体内作用.

主要方法:

在哺乳动物细胞中利用了使用选择性 (RUSH) 系统的保留.
在ER退出地点 (ERES) 调查了SHH与TMED10的局部化.
采用Drosophila翅膀形象性磁盘模型来研究TMED10同类 (贝泽) 的功能.

主要成果:

证明新合成的SHH遵循经典的分泌途径.
确定了TMED10作为一个内质网膜 (ER) 货物受体,促进ER-to-Golgi运输.
表明Rab6囊泡调解了高尔基到细胞的SHH表面贩运.
观察到TMED10耗尽导致显著的SHH释放缺陷.
证实了TMED10同源Baiser (Bai) 在 (Hh) 分泌和信号传递中在Drosophila中的作用.

结论:

TMED10对于货物特定的SHH从ER出口至关重要.
这项研究揭示了新合成的SHH分泌中的新合作伙伴.
这些发现对理解胚胎发育有潜在的影响.

关键词:

生物合成途径生物合成途径埃雷斯-埃雷斯是什么意思细胞内膜网膜的内细胞网膜.膜贩运活动膜贩运在p24蛋白质中.

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