超越自身氧化和脂氧化酶:植物油中的脂肪酸氧化产物
Elisabeth Koch1, Ariane Löwen1, Nadja Kampschulte1
1Chair of Food Chemistry, Faculty of Mathematics and Natural Sciences, University of Wuppertal, Gaussstrasse 20, Wuppertal 42119, Germany.
Journal of agricultural and food chemistry
|August 25, 2023
概括
这项研究表明,除了通常研究的氧脂肪酸外,在亚麻和大麻等植物油中更为丰富. 这些发现为评估植物油的质量和真实性提供了新的途径.
科学领域:
- 分析化学 分析化学
- 利皮多米克 (Lipidomics) 是一种消化剂.
- 植物生物化学 植物生物化学
背景情况:
- 关于植物油氧化的传统研究主要集中在从酸 (LA) 和α-酸 (ALA) 中衍生出来的特定基脂肪酸上.
- 以前的研究忽略了植物油中其他氧脂肪酸异构体的流行率.
研究的目的:
- 使用非向分析方法,识别和量化植物油中以前未经表征的基脂肪酸.
- 研究这些新型氧化脂肪酸的形成途径.
- 评估这些化合物作为石油质量和真实性的生物标志物的潜力.
主要方法:
- 非向的液体染色学-质谱学 (LC-MS) 和气体染色学-质谱学 (GC-MS) 分析.
- 使用真实的参考标准识别化合物,并根据质量和碎片化模式对未知的峰值进行表征.
- 研究涉及诸如CYP4F2和脂肪酸脱酶3等酶的形成途径.
主要成果:
- 发现了丰富的基脂肪酸,包括9,10,12,13和15-基十甲酸 (HODE) 和9和13-基十甲酸 (HOTrE).
- 对16-基-ALA和18-基-ALA的初步鉴定,以及对几个12-基-octadecadienoic酸异构体的特征鉴定,包括densipolic acid.
- 在冷压油中观察到的这些氧利平的度高达以前已知的氧脂肪酸的六倍.
结论:
- 基脂肪酸,特别是12-基异构体,是植物油的重要成分,比以前认为的更丰富.
- 涉及脂肪酸脱酶3和CYP4F2的酶途径有助于形成这些氧化脂质.
- 鉴定到的基脂肪酸可以作为评估植物油的质量和真实性的有价值指标.
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