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CircASPH 增强了外体棒,以促进结肠直肠癌中M2巨的极化

Yuting Zhang¹, Jiakun Guo², Liyin Zhang³

¹Department of Gastroenterology, the First Affiliated Hospital of University of Science and Technology of China (USTC), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, 230001, Anhui, China.

Inflammatory bowel diseases

|August 25, 2023

概括

循环RNAASPH (circASPH) 通过增强外体STING来促进结直肠癌 (CRC),这推动了M2巨的两极化. 向circASPH可能为CRC患者提供新的治疗策略.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

外体介导瘤微环境 (TME) 通信,影响癌症的进展.
循环RNAs (circRNAs) 是结直肠癌 (CRC) 的新兴调节剂,但它们的作用尚未完全理解.
在CRC中circRNA亚斯巴酸β-酶 (circASPH) 的特定功能及其与外体介导的TME的联系需要研究.

研究的目的:

研究circASPH在结直肠癌 (CRC) 进展中的作用.
探索circASPH和外体介导瘤微环境 (TME) 之间的相关性.
阐明CRC中circasph的潜在分子机制.

主要方法:

定量实时PCR测量CRC样本和细胞系中的circasph表达.
在体外和体内实验中评估circASPH缺乏对CRC细胞恶性瘤和瘤生长的功能影响.
共同培养试验和分子分析,以调查circASPH,巨细胞和干扰素基因外体刺激器 (STING) 之间的相互作用.

主要成果:

在CRC组织和细胞系中,circasph被显著上调.
循环ASPH缺乏抑制了CRC细胞恶性瘤,并通过增强抗瘤免疫力来抑制瘤生长.
circASPH通过与IGF2BP2相互作用,通过对外体STING进行上调来促进M2巨细胞的两极化,以稳定STING mRNA.

结论:

在CRC TME中,circASPH通过外体STING促进M2巨细胞两极分化,从而起到关键的调解作用.
circASPH在加速CRC进展方面发挥着重要作用.
circASPH代表了结直肠癌治疗的潜在治疗标.

关键词:

刺痛是一种刺痛.在CircASPH中使用.结肠直肠癌是什么意思外基因组是外基因组中的一个.巨细胞的两极分化

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