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向脉核的纳米载体促进了基于米尔纳的治疗方法,用于脊椎间盘退化

Zhonghui Chen^1,2,3, Zhong Liao^1,2, Ming Liu⁴

¹Orthopaedic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian, 350000, China.

Advanced healthcare materials

|August 25, 2023

概括

微RNA-22-3p (miR-22-3p) 在椎间盘退化 (IDD) 中至关重要. 在IDD模型中抑制miR-22-3p可以缓解退化,这表明潜在的miRNA治疗腰部疼痛.

科学领域:

分子生物学分子生物学
生物化学生物化学
再生医学是一种再生医学.

背景情况:

椎间盘退化 (IDD) 是腰部疼痛的主要原因,需要对其潜在的分子机制进行研究,以开发有效的治疗方法.
微RNAs (miRNAs) 越来越被认为是包括IDD在内的退行性疾病的关键调节者.

研究的目的:

阐明miR-22-3p在椎间盘退化 (IDD) 病变发生过程中的作用.
通过纳米粒子输送系统,研究针对miR-22-3p的治疗潜力,用于IDD治疗.

主要方法:

微RNA微阵列分析,体内 (淘汰赛小鼠) 和体内实验.
生物信息分析以确定miR-22-3p目标和信号通路 (SIRT1,JAK1/STAT3).
路西法酶记者测定,西部斑点分析和纳米粒子介导的miRNA抑制剂/模仿剂在IDD模型中的输送.

主要成果:

miR-22-3p淘汰赛小鼠表现出显著降低的组织学分数,表明具有保护作用.
鉴定出miR-22-3p是向SIRT1的关键调节器,SIRT1调节IDD中的JAK1/STAT3信号通路.
纳米粒子输送的miR-22-3p抑制剂缓解了IDD,而模仿剂在体内加重了这种情况.

结论:

miR-22-3p在脊椎间盘退化的分子调节中起着至关重要的作用.
针对miR-22-3p,特别是通过纳米粒子介导的抑制剂,为IDD和相关的腰部疼痛提供了一个有前途的治疗策略.
这项研究支持开发用于治疗IDD的miRNA特异性药物.

关键词:

的 IDD IDD 的 IDD.微RNAs 是一个微型RNA.纳米颗粒是一种纳米粒子.它的核是脉冲的脉冲.

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