在迁移过程中保持皮质索马托斯塔丁内部神经元亚型认同的转录性维持
Hermany Munguba1, Kasra Nikouei1, Hannah Hochgerner1
1Laboratory of Molecular Neurobiology, Department Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden.
Neuron
|August 25, 2023
概括
在迁移过程中,Sox6对于维持体静止素内部神经元亚型身份至关重要. 它的去除破坏了亚型的发展,突出了在神经元身份维护中需要活跃的转录程序的需要.
科学领域:
- 神经科学是一个神经科学.
- 发展生物学 发展生物学
- 遗传学 是一个遗传学.
背景情况:
- 皮层内部神经元的身份是建立后细胞周期的退出.
- 具体内部神经元亚型的预先建立和维持仍然不清楚.
- 了解子类型的维护对于电路集成至关重要.
研究的目的:
- 为了研究Sox6在维持体静止素 (Sst+) 内核神经元亚型认同中的作用.
- 在电路集成之前,确定Sox6是否需要预先建立的亚型标识.
主要方法:
- 在迁移的Sst+内部神经元中,Sox6 (Sox6-cKO) 的条件淘汰.
- 在青少年小鼠中的分子亚型标记物的分析.
- 对Sox6-cKO细胞的电生理学评估.
- 在胚胎日检查亚型身份 18.5.5.
主要成果:
- 在迁移的Sst+内部神经元中,Sox6的有条件去除导致了八种分子亚型中的五种几乎不存在.
- Sox6-cKO细胞表现出电生理成熟度,并表达了广泛的Sst+内部神经元基因.
- 在皮质整合之前,可以推断亚型的身份,这表明了维护缺陷.
- 在产后第7天的Sox6去除没有影响成熟亚型标记物表达.
结论:
- 在神经元迁移期间,Sox6对于维持Sst+内部神经元亚型身份至关重要.
- 亚型身份维护需要活跃的转录程序,而不仅仅是初始建立.
- 这些发现揭示了在皮层中控制内部神经元多样性的分子机制.
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