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相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Introduction to Enzyme Kinetics01:19

Introduction to Enzyme Kinetics

20.2K
Enzyme kinetics studies the rates of biochemical reactions. Scientists monitor the reaction rates for a particular enzymatic reaction at various substrate concentrations. Additional trials with inhibitors or other molecules that affect the reaction rate may also be performed.
The experimenter can then plot the initial reaction rate or velocity (Vo) of a given trial against the substrate concentration ([S]) to obtain a graph of the reaction properties. For many enzymatic reactions involving a...
20.2K
Enzyme Kinetics01:19

Enzyme Kinetics

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Enzymes speed up reactions by lowering the activation energy of the reactants. The speed at which the enzyme turns reactants into products is called the rate of reaction. Several factors impact the rate of reaction, including the number of available reactants. Enzyme kinetics is the study of how an enzyme changes the rate of a reaction.
Scientists typically study enzyme kinetics with a fixed amount of enzyme in the controlled environment of a test tube. When more reactant, or substrate, is...
97.2K
Mechanical Protein Functions01:58

Mechanical Protein Functions

5.0K
Proteins perform many mechanical functions in a cell. These proteins can be classified into two general categories- proteins that generate mechanical forces and proteins that are subjected to mechanical forces. Proteins providing mechanical support to the structure of the cell, such as keratin, are subjected to mechanical force, whereas proteins involved in cell movement and transport of molecules across cell membranes, such as an ion pump, are examples of generating mechanical force. 
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Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

6.9K
Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein....
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Protein-Drug Binding: Mechanism and Kinetics01:16

Protein-Drug Binding: Mechanism and Kinetics

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Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
Various forces drive these interactions, including hydrogen bonds, hydrophobic interactions, ionic bonds, electrostatic interactions, and van der Waals forces. These bonds enable drugs to bind to specific sites on proteins,...
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相关实验视频

Updated: Jul 18, 2025

The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis
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The Importance of Correct Protein Concentration for Kinetics and Affinity Determination in Structure-function Analysis

Published on: March 17, 2010

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K-Pro:蛋白质和突变物的动力学数据

Paola Turina1, Piero Fariselli2, Emidio Capriotti1

  • 1Department of Pharmacy and Biotechnology (FaBiT), University of Bologna, Via F. Selmi 3, 40126 Bologna, Italy.

Journal of molecular biology
|August 25, 2023
PubMed
概括

K-Pro是一个新的数据库,收集单体蛋白质的实验性蛋白质折叠动态数据. 本资源有助于了解蛋白质折叠机制和由错误折叠的蛋白质引起的人类疾病.

科学领域:

  • 生物化学和分子生物学
  • 结构生物学 结构生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 蛋白质折叠对于蛋白质功能和理解表型的遗传联系至关重要.
  • 错误折叠的蛋白质与各种人类疾病有关,需要研究折叠机制.
  • 关于蛋白质折叠的实验动力学和热力学数据对于这项研究至关重要.

研究的目的:

  • 介绍K-Pro,一个用于收集和存储蛋白质折叠实验动态数据的新型数据库.
  • 为研究蛋白质折叠动力学和热力学的研究人员提供全面的资源.
  • 促进对蛋白质折叠机制及其与人类疾病的关系的理解.

主要方法:

  • 开发一个专门的数据库,K-Pro,用于对具有双态折叠机制的单体蛋白的实验动力学数据.
  • 从62个蛋白质 (65个结构) 中整理和存储了1529个记录,包括折叠/展开率,坦福德的β和φ值.
  • 集成可用的热力学参数,并与外部数据库 (PDB,UniProt,PubMed) 进行交叉链接.

主要成果:

  • K-Pro 存储了大量的动力学数据,包括对数折叠/展开率,坦福德的β值和62种蛋白质的φ值.
  • 数据库包含1529个记录,包括可用的热力学数据.
关键词:
折叠运动学 折叠运动学折叠率常数是指折叠率的常数.蛋白质折叠 蛋白质的折叠蛋白质变体的蛋白质变体过渡状态的过渡状态.

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Using In Vitro Fluorescence Resonance Energy Transfer to Study the Dynamics Of Protein Complexes at a Millisecond Time Scale
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  • 一个用户友好的界面允许轻松浏览,下载和可视化蛋白质和突变数据.

    • 结论:

    • K-Pro是蛋白质折叠研究的一个有价值的,免费可访问的资源.
    • 该数据库支持研究蛋白质折叠动力学和热力学,有助于调查蛋白质错误折叠疾病.
    • K-Pro的集成数据和用户友好的界面提高了科学界的可访问性和实用性.