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来自珍珠小米的不溶于水的多糖的结构分析,并评估其前生物活性

Susmita Mondal¹, Shivangi Agrawal¹, Abinaya Balasubramanian¹

¹Department of Biotechnology, Indian Institute of Technology Kharagpur, West Midnapore, West Bengal 721302, India.

International journal of biological macromolecules

|August 25, 2023

概括

珍珠小米多糖 (PMG) 显示了益生菌的潜力,促进有益的肠道细菌. 这种葡萄糖可以作为食品和制药中的功能成分,以支持健康.

科学领域:

食品科学与技术食品科学与技术
微生物学微生物学
生物化学生物化学

背景情况:

流行病学研究将益生菌摄入与降低慢性疾病风险联系起来.
珍珠小米是一种经济上可行的新型益生菌多糖的来源.
了解多糖的结构和功能是开发功能性食品的关键.

研究的目的:

为了研究珍珠小米中水不溶性同聚糖 (PMG) 的化学结构.
为了评估PMG的益生菌性质.
探索PMG在食品和制药应用中的潜力.

主要方法:

使用总水解,甲基化分析和1D/2DNMR光谱学进行结构阐明.
通过唾液和胰腺α-amylase进行水解的评估.
通过使用Lactobacillus acidophilus和Lactobacillus brevis来确定前生物潜力.

主要成果:

PMG是一种分子量约为361kDa的葡萄糖,具有 (1→3) α-d-glucopyranosyl骨干.
在PMG中,唾液 (1.75%) 和胰腺 (1.99%) 的α-amylase的水解率较低.
在L. acidophilus (0.446) 和L. brevis (0.427) 中,PMG表现出了积极的益生菌评分.

结论:

阐明的结构和低消化性表明PMG的适用性作为一种益生菌.
PMG支持有益细菌的生长,表明了益生菌的有效性.
作为食品和制药行业的功能成分,PMG具有前景.

关键词:

葡萄糖是一种葡萄糖.这是NMR的NMR.珍珠小米是一种珍珠小米.益生菌益生菌益生菌结构结构是一个结构.

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