MYC2转录因子的部分脱敏化会改变与莉花酸信号元件的相互作用,并影响专门的新陈代谢
Xin Hou1, Sanjay Kumar Singh2, Joshua R Werkman2
1Department of Tobacco, College of Plant Protection, Shandong Agricultural University, Shandong Province Key Laboratory of Agricultural Microbiology, Tai'an 271018, China.
International journal of biological macromolecules
|August 25, 2023
概括
改变MYC2蛋白 (一种植物调节剂) 中的一个关键残留物可以增强与MED25的相互作用,并减少JAZ抑制. 这促进了烟草中的尼古丁产量,为天然产品的合成提供了一种新方法.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物生物化学 植物生物化学
- 代谢工程是代谢工程.
背景情况:
- MYC2是一个关键的bHLH转录因子,调节植物特种代谢和莉花信号传递.
- MYC2活动是由JASMONATE-ZIM-domain (JAZ) 抑制剂和MED25联合激活剂调节的.
- 在MYC2的JAZ相互作用域 (JID) 中保存残留物的作用及其对MED25相互作用的影响在很大程度上仍未被阐明.
研究的目的:
- 为了研究替代品在NtMYC2a的JID中保存的D128残留物的功能影响.
- 确定这些替代物如何影响与JAZ蛋白和MED25的相互作用.
- 评估D128替代剂对烟草中尼古丁生物合成和积累的影响.
主要方法:
- 用于产生各种NtMYC2a D128替代变体 (例如D128N,D128P) 的地方定向突变发生.
- 烟草毛的根培养被用来过度表达野生型和突变的NtMYC2a蛋白质.
- 进行了蛋白质与蛋白质相互作用测试,基因表达分析和尼古丁含量量定量.
- 使用结构建模和模拟来理解观察效应的机械基础.
主要成果:
- 该NtMYC2aD128N变种显示了对JAZ镇压的部分脱敏,以及与MED25的增强互动.
- 过度表达NtMYC2aD128N导致尼古丁通路基因的显著增加表达和更高的尼古丁积累.
- 替代NtMYC2aD128P取消了与JAZ和MED25的交互,影响了交易.
- 结构分析表明,D128替代改变了JID结合口袋的稳定性,解释了功能结果.
结论:
- 在NtMYC2a中保存的D128残留物在调节JAZ抑制和MED25联合激活方面发挥着关键作用.
- 特定的替代物,如D128N,可以被利用来增强二次代谢物生产,如尼古丁.
- 这项研究为设计植物天然产品生物合成途径提供了一种新的策略.
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