生物化学和遗传证据支持Fyv6作为Saccharomyces cerevisiae中的第二阶段拼接因子
Karli A Lipinski1, Katherine A Senn2, Natalie J Zeps2
1Department of Chemistry, University of Wisconsin-Madison, Madison, Wisconsin 53706, USA.
概括
Fyv6是一种酵母拼接因子,对外链结合至关重要,影响3'拼接部位的选择. 这项研究提供了支持Fyv6作为人类FAM192A.的功能同源的生化和遗传证据.
科学领域:
- 分子生物学分子生物学
- 基因表达规范 基因表达规范
- 单核生物基因表达的表达方式
背景情况:
- 前体mRNA (pre-mRNA) 拼接对于真核细胞基因表达至关重要,由拼接体催化.
- 人类蛋白质FAM192A最近被假设是参与第二个转化步骤 (外因子结合) 的剪接因子.
- Fyv6被提议作为FAM192A的潜在Saccharomyces cerevisiae同类物,但缺乏实验验证.
研究的目的:
- 以生化和遗传验证Fyv6作为酵母中的拼接因子.
- 为了研究Fyv6在拼接的外绑定步骤中的作用.
- 确定Fyv6是否影响结合部位选择,并确认其与人类FAM192A.的同质性.
主要方法:
- 用基本拼接因子删除FYV6的遗传相互作用分析.
- 在体内剪接试验中,使用带有修改内基因的记者基因进行了分析.
- 在体外剪接试验中,使用全细胞提取物进行了测试.
- 在记者基因和内源性转录中对3'拼接位 (SS) 选择的分析.
主要成果:
- 删除FYV6显示与关键拼接因子 (Prp8,Prp16,Prp22) 的遗传相互作用,并降低了体内拼接效率.
- 缺乏Fyv6的酵母提取物累积了拼接中间体,并在体外显示出受损的外链结合.
- 失去了Fyv6的功能改变了3'SS的使用在两个记者构造和内源的SUS1基因.
结论:
- Fyv6在酵母中起到拼接因子的作用,特别是在外结合阶段.
- Fyv6在调节3' SS选择方面发挥着作用.
- Fyv6很可能是酵母中人类FAM192A的功能和结构同类.
更多相关视频
07:00Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Published on: June 14, 2013
34.9K
05:44Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes
Published on: November 9, 2020
3.7K
相关概念视频
Protein Complexes with Interchangeable Parts
2.6K
Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
2.6K
RNA Splicing
56.5K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.5K
Pre-mRNA Processing: RNA Splicing
5.3K
5.3K
Alternative RNA Splicing
21.3K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.3K
Yeast Signaling
14.7K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
14.7K
Chromatin Structure and RNA Splicing
2.7K
2.7K
