铁酸盐和阿波转移素使红细胞细胞与来自血源性内皮质的β-环球蛋白成熟
Soo-Been Jeon1, Hyebin Koh2,3, A-Reum Han1
1CHA Advanced Research Institute, Bundang CHA Medical Center, CHA University, Seongnam, Kyunggi-do, 13488, South Korea.
NPJ Regenerative medicine
|August 25, 2023
概括
从人类多能干细胞 (PSC) 产生功能性的红细胞 (RBC) 对再生医学至关重要. 铁酸盐治疗在体外显著增强红细胞成熟和β-环球蛋白表达,为临床应用铺平了道路.
科学领域:
- 干细胞生物学 干细胞生物学
- 血液学 血液学 血液学
- 再生医学是一种再生医学.
背景情况:
- 从人类多能干细胞 (PSC) 产生功能性的红细胞 (RBC) 对细胞疗法和发育研究至关重要.
- 目前的方法面临着功能性红细胞效率低,由于缺乏生理微环境而导致成熟受损的挑战.
研究的目的:
- 开发一种简单,可复制的培养系统,从PSCs生成具有高效β-环球蛋白表达的红细胞.
- 调查铁酸盐在促进红细胞成熟和功能方面的作用.
主要方法:
- 使用确定的血源内皮 (HE) 建立了一个二维定义的培养系统.
- 应用铁酸盐治疗HE衍生浮动红细胞.
- 分析了细胞表面标记物 (CD45,CD71,CD235a) 和与β-环球蛋白和血红蛋白合成相关的基因表达.
主要成果:
- 铁酸盐治疗显著增加了CD45+CD71+CD235a+红细胞的数量.
- 培养的红细胞体表现出高的β-环球蛋白转录水平和合成和细胞循环基因的丰富.
- 在体内研究表明,这些红细胞细胞迅速成熟成为功能性的红细胞.
结论:
- 铁酸盐在定义的培养系统中有效促进红红细胞成熟和β-环球蛋白表达.
- 这种方法提供了一个稳定的协议,用于从PSC生成临床适用的红细胞.
- 研究结果表明,铁酸盐通过促进铁的吸收和利用来增强成熟.
相关概念视频
The Early Endosome: Endocytosis of Transferrin
3.3K
Essential proteins such as insulin or low-density lipoprotein (LDL) and micronutrients such as iron enter a eukaryotic cell through receptor-mediated endocytosis. Subsequently, the early endosomes fuse with the vesicles containing such receptor-ligand complexes and play a vital role in sorting the incoming ligands and receptors. While the ligands are either degraded inside the vesicle or released into the cytosol, their receptors are returned to the plasma membrane for further rounds of...
3.3K
Erythropoiesis
4.4K
Red blood cells (RBCs) transport oxygen to all body tissues. These cells survive only for 120 days and then need to be replenished. Erythropoiesis is the process of RBC production. In healthy individuals, erythropoiesis ensures all tissues are amply supplied with oxygen. In addition, blood loss due to injury leads to a drop in the physiological oxygen level that will cause erythropoiesis. Any defect in erythropoiesis leads to several physiological disorders, including thalassemia, anemia,...
4.4K
Lifecycle of Erythrocytes
2.0K
Erythrocytes, also known as red blood cells, constantly move through blood capillaries. As a result, they damage their plasma membrane due to the continuous friction. Typically, after 100 to 120 days, erythrocytes become rigid and fragile as they wear out. As they pass through small vessels in the spleen and liver, they can get trapped and break apart into fragments.
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups....
The resident phagocytic macrophages deal with these damaged cells by engulfing them and separating their globin and heme groups....
2.0K
Role of Hematopoietic Growth Factors
1.4K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
Thrombopoietin (TPO), mainly released by the liver,...
1.4K
Factors Affecting Erythropoiesis
3.3K
The cardiovascular system regulates the number of erythrocytes in the bloodstream to ensure optimal oxygen transport. It also prevents over-proliferation of these cells, which helps to maintain blood viscosity and flow rate.
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
Several factors influence the erythrocyte production rate, with tissue oxygen level being among the most critical. Intense exercise or high altitudes can cause tissue hypoxia, which triggers the kidneys to release more erythropoietin (EPO) into the bloodstream.
EPO then...
3.3K
Hematopoiesis
5.4K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.4K


