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Updated: Jul 18, 2025

Gel-seq: A Method for Simultaneous Sequencing Library Preparation of DNA and RNA Using Hydrogel Matrices
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一个高效的方案从单链DNA编制图书馆

Fumihito Miura1, Hideaki Kanzawa-Kiriyama2, Osamu Hisano3,4

  • 1Department of Biochemistry, Kyushu University Graduate School of Medical Sciences, 3-1-1 Maidashi, Higashi-Ku, Fukuoka, 812-8582, Japan. fumihito@med.kyushu-u.ac.jp.

Scientific reports
|August 25, 2023
PubMed
概括

我们开发了一种新的TACS-TOPO方法,用于高效的单链DNA (ssDNA) 测序库准备. 这种技术可以提高具有挑战性的样本的产量,例如古代DNA和无细胞DNA.

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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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