多宿主感染和遗传学上多样化的血统塑造了Staphylococcus aureus的重组和基因库动态
Stephanie S R Souza1, Joshua T Smith2,3, Spencer A Bruce4
1Department of Biological Sciences, University at Albany, State University of New York, Albany, NY, USA. ssrdesouza@albany.edu.
BMC microbiology
|August 25, 2023
概括
黄金葡萄球菌的遗传重组促使其适应不同宿主. 频繁的基因共享,即使是罕见的血统,也塑造了其适应性基因库,这对于理解其传播和控制至关重要.
科学领域:
- 微生物学 微生物学
- 进化生物学 进化生物学
- 基因组学就是基因组学.
背景情况:
- 黄金葡萄球菌在多种宿主物种中表现出适应性,但这种通用生活方式的遗传驱动因素尚未完全理解.
- 黄金菌的本地种群经常与不同的宿主物种相互作用,可能有助于适应.
- 这项研究调查了S. aureus与美国东北部人类和动物疾病病例的基因组差异.
研究的目的:
- 为了阐明人类和动物衍生的金黄色葡萄球菌分离物之间的基因组差异.
- 确定导致S. aureus感染多个宿主的能力的遗传和进化因素.
- 为了告知控制S. aureus殖民,感染和传播的战略.
主要方法:
- 多位置序列类型 (MLST) 用于识别序列类型 (ST).
- 在437个S. aureus分离物上进行了种群基因组分析.
- 分析了辅助基因组含量,抗微生物耐药性,病毒性基因和重组事件.
主要成果:
- MLST确定了75种已知的ST,其中ST5,ST8和ST30占主导地位.
- 辅助基因组含量在各个血统之间有所不同,抗微生物耐药性和毒性基因的分布差异基于宿主起源和ST.
- 在765个基因中发现了1,912个重组事件,人类和动物分离物之间的同源重组是可比的;低频STs是显著的DNA捐赠者.
结论:
- 在共同循环的金黄色菌株中,包括罕见的,重组模式塑造了当地的基因库和与宿主相关的遗传变异.
- 频繁的重组,特别是在毒性基因中,有助于S. aureus的多宿主适应性.
- 在多种不同的宿主中对金黄色细菌的持续监测对于在"一个健康"框架内有效的控制策略至关重要.
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