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在老化过程中,皮下脂肪组织的改变与甲状腺激素信号传递相关

Wen-Na Zhang¹, Hao Zhu¹, Zhen-Wu Ma¹

¹Division of Endocrinology, the First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.

BMC medical genomics

|August 25, 2023

概括

皮下脂肪组织 (SAT) 的老化显示了线粒体功能和基因表达的改变,可能是由于甲状腺激素不敏感. 这项研究确定了七个关键基因,这些基因与与年龄相关的SAT功能障碍和代谢疾病风险有关.

科学领域:

代谢性疾病研究研究.
生物老龄化生物学
脂肪组织研究研究脂肪组织研究.

背景情况:

皮下脂肪组织 (SAT) 的功能变化发生在衰老的早期,导致代谢疾病.
与年龄相关的SAT功能障碍的机制及其对代谢健康的影响仍然不清楚.

研究的目的:

为了研究人类SAT中与年龄相关的功能变化.
为了确定关键的基因和SAT衰老背后的分子机制.
探索甲状腺激素在SAT衰老中的作用.

主要方法:

转录组测序 (RNA-seq) 和对转化酶可访问的高吞吐量测序 (ATAC-seq) 染色质的测定在人类SAT.
基因表达分析和功能注释 (GO,KEGG).
鼠标脂肪细胞模型与甲状腺激素刺激,RT-PCR,氧气消耗测试和免疫光染色.

主要成果:

在老年人SAT中,RNA-seq发现了331个上调和349个下调的基因.
七个基因 (NCF1,NLRP3,DUOX1,IFI30,P2RX1,P2RX1,P2RX6,PRODH) 显示出与年龄相关的表达变化.
线粒体功能发生变化,老年人SAT中ROS积累增加,这些基因对小鼠的甲状腺激素作出反应.

结论:

老年SAT表现出显著的功能变化,特别是在线粒体中,可能与甲状腺激素信号不敏感有关.
通过甲状腺激素调节的7个新型基因被确定,与与年龄相关的SAT功能障碍,线粒体损伤和ROS积累有关.

关键词:

衰老的衰老衰老的衰老染色体的可访问性染色体的可访问性线粒体中的线粒体.皮下脂肪组织 (SAT)甲状腺激素是甲状腺激素的一种.

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