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相关概念视频

Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
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Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
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DefinitionComputed Tomography (CT) of the genitourinary (GU) tract is a non-invasive imaging modality that utilizes X-rays and computer processing to generate detailed cross-sectional images of the urinary system, encompassing the kidneys, ureters, bladder, and adjacent structures such as the adrenal glands.PurposeCT scans of the GU tract serve several diagnostic and therapeutic purposes, including:Diagnosis of Urinary Tract Diseases: Detects kidney stones, tumors, cysts, and congenital...
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相关实验视频

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工作聪明,不努力:数组断层扫描如何有助于增加超结构数据输出.

Irina Kolotuev1

  • 1Electron Microscopy Facility, University of Lausanne, Lausanne, Switzerland.

Journal of microscopy
|August 26, 2023
PubMed
概括

阵列断层扫描 (AT) 为生物样品提供了多功能超结构分析,克服了传输电子显微镜 (TEM) 的局限性. 这种方法可以为研究和诊断提供详细的成像.

科学领域:

  • 细胞生物学 细胞生物学
  • 显微镜技术 显微镜技术
  • 生物技术是生物技术.

背景情况:

  • 几十年来,传输电子显微镜 (TEM) 是细胞生物学的一个基石.
  • 卷电子显微镜的进步,如串行块面和聚焦离子束扫描电子显微镜,已经增强了超结构分析.
  • 阵列断层扫描 (AT) 作为一种用于树脂嵌入样品的序列成像技术而出现.

研究的目的:

  • 突出阵列断层扫描 (AT) 的功能和多功能性.
  • 证明AT在克服传统传输电子显微镜 (TEM) 的局限性方面的潜力.
  • 展示AT在各种生物研究和诊断环境中的应用性.

主要方法:

  • 在大型支架上对树脂嵌入的样本部分进行序列图像采集.
  • 用多个光标签进行免疫标记,并与超结构观察相结合.
  • 用扫描电子显微镜 (SEM) 来生成和成像连续部分进行3D重建.

主要成果:

  • 在传统的TEM中,AT方便了难以或不可能完成的任务.
  • 该技术适用于用于选择部分或体积分析的各种生物样本.
  • AT与形态分析,多重免疫标记,免疫黄金标记以及相关光和电子显微镜兼容.
关键词:
这就是SEM SEM.阵列断层扫描 阵列断层扫描血液细胞 细胞 血细胞诊断 vEM 进行测试组织学 组织学 组织学模型生物模型生物.卷电子显微镜的体积电子显微镜.

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结论:

  • 阵列断层扫描 (AT) 为超结构分析提供了一种多功能且简单的方法.
  • 它与多种标签技术和工作流程的兼容性使其对基础研究具有价值.
  • 该技术的适应性使其成为一种潜在的诊断工具,简化了常规程序.