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工作聪明,不努力:数组断层扫描如何有助于增加超结构数据输出

Irina Kolotuev¹

¹Electron Microscopy Facility, University of Lausanne, Lausanne, Switzerland.

Journal of microscopy

|August 26, 2023

概括

阵列断层扫描 (AT) 为生物样品提供了多功能超结构分析,克服了传输电子显微镜 (TEM) 的局限性. 这种方法可以为研究和诊断提供详细的成像.

科学领域:

细胞生物学细胞生物学
显微镜技术显微镜技术
生物技术是生物技术.

背景情况:

几十年来,传输电子显微镜 (TEM) 是细胞生物学的一个基石.
卷电子显微镜的进步,如串行块面和聚焦离子束扫描电子显微镜,已经增强了超结构分析.
阵列断层扫描 (AT) 作为一种用于树脂嵌入样品的序列成像技术而出现.

研究的目的:

突出阵列断层扫描 (AT) 的功能和多功能性.
证明AT在克服传统传输电子显微镜 (TEM) 的局限性方面的潜力.
展示AT在各种生物研究和诊断环境中的应用性.

主要方法:

在大型支架上对树脂嵌入的样本部分进行序列图像采集.
用多个光标签进行免疫标记,并与超结构观察相结合.
用扫描电子显微镜 (SEM) 来生成和成像连续部分进行3D重建.

主要成果:

在传统的TEM中,AT方便了难以或不可能完成的任务.
该技术适用于用于选择部分或体积分析的各种生物样本.
AT与形态分析,多重免疫标记,免疫黄金标记以及相关光和电子显微镜兼容.

结论:

阵列断层扫描 (AT) 为超结构分析提供了一种多功能且简单的方法.
它与多种标签技术和工作流程的兼容性使其对基础研究具有价值.
该技术的适应性使其成为一种潜在的诊断工具,简化了常规程序.

关键词:

这就是SEM SEM.阵列断层扫描阵列断层扫描血液细胞细胞血细胞诊断 vEM 进行测试组织学组织学组织学模型生物模型生物.卷电子显微镜的体积电子显微镜.

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