局部麻醉剂对中性粒细胞的影响在不同隔离技术的背景下
Sara Sixt1, Michael Gruber1, Gesche Kolle1
1Department of Anesthesiology, University Hospital Regensburg, 93042 Regensburg, Germany.
Biomedicines
|August 26, 2023
概括
局部麻醉剂 (LAs) 影响多态核中性粒细胞 (PMN) 的功能,如迁移和ROS产生. 布皮瓦卡因和利多卡因,但不是levobupivacaine或ropivacaine,在体外减少了NETosis.
科学领域:
- 免疫学 免疫学 免疫学
- 药理学 药理学是指药理学的学科.
背景情况:
- 多态核中性粒细胞 (PMNs) 在疾病和手术后变化中起着至关重要的作用.
- 已知局部麻醉剂 (LA) 影响PMN功能,但效果因隔离方法而异.
研究的目的:
- 调查选定的LA对PMN功能的影响.
- 为了比较使用新型隔离方法与传统技术对LAs的影响.
主要方法:
- 使用凝素沉积,低压溶解或密度梯度离心来分离PMN.
- 孤立的PMN暴露于不同度的布皮瓦卡因,勒沃布皮瓦卡因,利多卡因或罗皮瓦卡因.
- 活细胞成像和流细胞测量评估了迁移,ROS生产,NETosis和抗原表达.
主要成果:
- LAs抑制了PMN化学反应和ROS的产生.
- 布皮瓦卡因和利多卡因显著减少了NETosis时间,而勒沃布皮瓦卡因和罗皮瓦卡因没有.
- 隔离方法影响了PMN生存时间和迁移持续时间.
结论:
- 选择的LA可以在体外调节PMN功能,包括迁移,ROS产生和NETosis.
- 选择LA和隔离方法影响观察到的PMN反应.
- 精心选择LAs可能对体外PMN行为产生短期影响.
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