相关实验视频

Updated: Jul 18, 2025

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

A Protocol for Using Gene Set Enrichment Analysis to Identify the Appropriate Animal Model for Translational Research

Published on: August 16, 2017

用基因组和生物信息学方法评估人类疾病研究的五种哺乳动物模型

Sankarasubramanian Jagadesan¹, Pinaki Mondal², Mark A Carlson^1,2

¹Department of Genetics, Cell Biology and Anatomy, University of Nebraska Medical Center, Omaha, NE 68198, USA.

|August 26, 2023

概括

为人类疾病研究选择合适的动物模型至关重要. 这项研究比较了五种哺乳动物模型和人类的遗传相似性,确定哪些模型最适合基于保存的基因和相关的单核酸多态 (SNP) 的特定疾病研究.

科学领域:

进行比较的基因组学.
翻译医学是一种翻译医学.
动物模型开发动物模型开发

背景情况:

评估人类疾病研究的动物模型需要评估遗传,表观遗传和代谢相似性.
在多个层面上经常缺乏一致的数据,阻碍了最佳模型选择.
基因组测序的进步使得跨物种的基因组比较能够进行模型适应性.

研究的目的:

为了比较五种哺乳动物模型 ( rhesus macaque, marmoset,猪,老鼠,老鼠) 的编码序列 (CDS) 与人类的 CDS.
在这些物种中,在这些保护区域内识别保存的CDS并绘制与人类疾病相关的单核酸多态 (SNP).
根据基因组相似性和SNP关联,评估这些动物模型对于研究特定人类疾病的适用性.

主要方法:

在人类和五种哺乳动物模型 ( rhesus macaque, marmoset,猪,老鼠,老鼠) 之间对编码序列 (CDS) 的比较分析.
在所有物种中使用序列相似性识别保存的CDS.
从保存的CDS到每个模型物种,绘制与人类疾病相关的单核酸多态 (SNP).

主要成果:

在五种动物模型和人类中,确定了10,316个保存的CDS.
发现了保存的CDS与人类疾病之间的特定物种关联.
rhesus 表现出最高数量的模型特定的人类疾病关联.
马尔莫塞特模型显示,基于与疾病相关的含有SNP的基因,对研究行为和心血管疾病具有很强的适应性.

结论:

基因组相似性评估为选择适合人类疾病研究的动物模型提供了一个框架.
马尔莫塞特和 rhesus macaque 模型在特定疾病研究领域表现出明显的优势.
这种比较基因组方法有助于研究人员选择最相关的动物模型,提高翻译研究的效率.

关键词:

国家公用事业单位是国家公用事业单位.国家统一计划 (SNP) 是一个国家统一计划.动物模型动物模型人类疾病人类疾病马尔莫塞特 - 马尔莫塞特 - 马尔莫塞特 - 马尔莫塞特动物动物动物序列的相似性序列的相似性

更多相关视频

In Vivo Modeling of the Morbid Human Genome using Danio rerio

In Vivo Modeling of the Morbid Human Genome using Danio rerio

Published on: August 24, 2013

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

Navigating MARRVEL, a Web-Based Tool that Integrates Human Genomics and Model Organism Genetics Information

Published on: August 15, 2019

相关概念视频

Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

Evolutionary Relationships through Genome Comparisons

Evolutionary Relationships through Genome Comparisons

Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...

Genomics

Genomics

Genomics is the science of genomes: it is the study of all the genetic material of an organism. In humans, the genome consists of information carried in 23 pairs of chromosomes in the nucleus, as well as mitochondrial DNA. In genomics, both coding and non-coding DNA is sequenced and analyzed. Genomics allows a better understanding of all living things, their evolution, and their diversity. It has a myriad of uses: for example, to build phylogenetic trees, to improve productivity and...