相关实验视频

Updated: Jul 18, 2025

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform

Published on: November 9, 2017

细胞因子多态性在慢性炎症性脱线性多神经病症患者中

Ivo Bozovic¹, Vladimir Perovic², Ivana Basta³

¹Neurology Clinic, University Clinical Center of Serbia, 11000 Belgrade, Serbia.

|August 26, 2023

概括

介素-10 (IL-10) 和介素-6 (IL-6) 基因中的单核酸多态 (SNPs) 可能与慢性炎症性脱髓化多神经病 (CIDP) 相关. 这些遗传变异也可能影响CIDP患者的疾病严重程度和2型糖尿病风险.

科学领域:

免疫遗传学免疫遗传学
神经免疫学神经免疫学
自免疫性疾病自免疫性疾病

背景情况:

慢性炎症性脱髓化多神经病变 (CIDP) 的发病包括免疫反应和细胞因子的产生.
单核酸多态 (SNP) 可以影响细胞因子基因表达,可能影响自身免疫性疾病的发展.
之前的研究还没有在CIDP患者中研究细胞因子基因SNP.

研究的目的:

评估细胞因子基因中的功能性SNP与CIDP发生和特征的关联.
调查特定SNP与疾病严重程度或2型糖尿病 (T2D) 等并发症之间的潜在联系.

主要方法:

量化PCR (qPCR) 用于分析IL-10,IL-6,TNF,IL-12B,IFN-γ,GM-CSF和IL-17F基因中的SNP.
一组88名CIDP患者和486名健康对照 (HC) 进行了基因定型.
进行了统计分析,以比较群体之间的基因型和等位基因频率.

主要成果:

在IL-10促进体SNP (rs1800896) 和CIDP发生之间发现了关联,患者与HC的基因型频率不同.
特定的IL-10SNP (rs1800871,rs1800872) 在CIDP患者中显示出较低的等位基因频率.
某些IL-10SNP的携带者与较轻的疾病有关 (低INCAT),而IL-6SNP (rs1800795) 在患者中更频繁,并且与T2D相关.
在CIDP患者中,IL-6 rs1800795 GG基因型更频繁,G载体患T2D的频率更高.

结论:

在IL-10和IL-6基因中的特定SNP显示了与CIDP的潜在关联.
这些遗传变异也可能与疾病严重程度和CIDP患者2型糖尿病的存在有关.
由于样本规模有限,需要进行更大规模的多中心研究来证实这些发现.

关键词:

慢性炎症性脱髓化的多神经病变.细胞因子细胞因子单核酸多形态的单核酸多形态

更多相关视频

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Induction of Experimental Autoimmune Encephalomyelitis in Mice and Evaluation of the Disease-dependent Distribution of Immune Cells in Various Tissues

Published on: May 8, 2016

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

High-resolution Melting PCR for Complement Receptor 1 Length Polymorphism Genotyping: An Innovative Tool for Alzheimer's Disease Gene Susceptibility Assessment

Published on: July 18, 2017

相关概念视频

Comparing Copy Number Variations and SNPs

Comparing Copy Number Variations and SNPs

Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...