相关实验视频

Updated: Jul 18, 2025

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

Preparation and Characterization of Individual and Multi-drug Loaded Physically Entrapped Polymeric Micelles

Published on: August 28, 2015

基于SDS的新型聚电解质多核纳米载体用于帕克利塔克塞尔的输送-合成,表征和对乳腺癌细胞的活性

Marzena Szwed¹, Sylwia Michlewska², Katarzyna Kania³

¹Department of Medical Biophysics, Institute of Biophysics, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143 St, 90-236 Lodz, Poland.

|August 26, 2023

概括

新型纳米载体有效地输送帕克利塔塞尔 (PTX),保持其杀死癌症的能力. 这些基于SDS的多电解质纳米载体显示出改善化疗的前景,特别是三阴性乳腺癌.

科学领域:

纳米技术纳米技术
材料科学材料科学材料科学
在瘤学瘤学.

背景情况:

疏水性抗癌药物面临患者分布差,限制化疗疗效.
帕克利塔克塞尔 (PTX) 是一种关键的化疗剂,可以改善其输送.

研究的目的:

评估带有帕克利塔塞尔的SDS基聚电解质纳米载体 (NCs) 的抗瘤特性.
确认封装PTX是否保持其细胞毒性活性.
为了研究这些新型纳米载体的药物传递潜力.

主要方法:

一层一层 (LbL) 组装以创建基于SDS的聚电解质多核纳米载体 (NC).
在实验室中对MCF-7,MDA-MB-231 (癌症) 和HMEC-1 (非癌症) 细胞系进行细胞毒性测定.
使用共聚焦显微镜,光谱测量,光谱测量,流细胞测量和RT PCR进行亡特征分析.

主要成果:

载有PTX的NCs表现出与自由PTX相比较的细胞毒性,特别是针对三阴性乳腺癌细胞.
SDS/PLL/PTX和SDS/PLL/PGA/PTX配方显著抑制了DNA合成.
带有PTX的NCs诱导了亡,并上调了与亡相关的关键基因 (Bax,AIF,cytochrome-c,caspase-3).

结论:

基于SDS的多电解质多核NCs是有效的载体,可以传递帕克利塔克塞尔.
这些纳米载体保留了PTX的细胞毒性,并可以触发亡.
开发的纳米载体显示出各种抗癌治疗应用的巨大潜力.

关键词:

在 EPR 效应中,EPR 效应是:灭症 (apoptosis) 是一种死亡的过程.纳米囊是一种纳米囊.纳税人就是纳税人.

更多相关视频

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

Preparation and Characterization of Lipophilic Doxorubicin Pro-drug Micelles

Preparation and Characterization of Lipophilic Doxorubicin Pro-drug Micelles

Published on: August 2, 2016

相关概念视频

Drugs that Stabilize Microtubules

Drugs that Stabilize Microtubules

Microtubules are dynamic structures that undergo cycles of catastrophe and rescue. The microtubules play a central role in cell division by forming the spindle apparatus for segregating the chromosomes. This makes them ideal targets for regulating dividing cells in tumors and malignant cancer cells. Microtubule stabilizing drugs help stabilize the microtubule formation and promote its polymerization. Paclitaxel was the first microtubule stabilizing agent used as anticancer drug in chemotherapy...