内质网膜是植物质的侵略和宿主植物防御之间的关键战场
Junichi Inaba1, Bo Min Kim1, Yan Zhao1
1Molecular Plant Pathology Laboratory, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Beltsville, MD 20705, USA.
Cells
|August 26, 2023
概括
马紫色顶部植物等离子体感染会破坏番茄植物内质网膜 (ER) 功能. 激活ER压力和展开的蛋白质反应 (UPR) 有助于控制植物质的增殖.
科学领域:
- 植物病理学 植物病理学
- 分子植物-微生物相互作用
- 细胞生物学 细胞生物学
背景情况:
- 植物质体是具有有限的代谢能力的有义务的细胞内植物病原体.
- 细胞内膜网膜 (ER) 对于宿主细胞平衡至关重要,也是病原体的点.
- 马紫色顶部 (PPT) 植物质会导致大量的作物损失.
研究的目的:
- 调查PPT植物等离子体感染对番茄植物ER的影响.
- 阐明ER应激和未折叠蛋白反应 (UPR) 在植物质与宿主相互作用中的作用.
- 探索针对ER的潜在治疗策略.
主要方法:
- 对焦显微镜和传输电子显微镜 (TEM) 可视化ER结构和蛋白质定位.
- 定量实时PCR (qRT-PCR) 用于评估ER应激/UPR激活和植物质标位.
- 实验性操纵ER应激通路和特定的ER居民蛋白.
主要成果:
- PPT植物质体感染诱导了ER压力,其特征是异常的蛋白质积累和破坏的ER网络.
- 植物质体与ER压力标志物结合在一起,表明ER恒温干扰和病原体殖民之间的联系.
- ER压力/UPR激活与植物质量位负相关,ER压力的实验诱导降低了病原体负载.
- 抑制ER定居蛋白BiP增加了植物质量位.
结论:
- ER是植物质体与宿主相互作用的战场,植物质体利用ER功能,宿主激活防御反应.
- ER压力和UPR激活是宿主防御植物质体感染的关键组成部分.
- 准ER压力路径是一个有希望的策略,用于管理植物等离子体疾病.
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