相关实验视频

Updated: Jun 14, 2026

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury

Ovine Lumbar Intervertebral Disc Degeneration Model Utilizing a Lateral Retroperitoneal Drill Bit Injury

Published on: May 25, 2017

高压氧干预对退化的椎间盘的影响:从分子机制到动物模型

Song-Shu Lin^1,2,3, Steve W N Ueng^1,3,4, Kowit-Yu Chong^3,5

¹Department of Orthopaedic Surgery, Chang Gung Memorial Hospital, Taoyuan 333, Taiwan.

|August 26, 2023

概括

高压氧疗法 (HBO) 调节微RNA-107 (miR-107) 抑制Wnt3a/β-catenin信号传输,提供对椎间盘 (IVD) 退化的保护作用.

科学领域:

生物医学工程生物医学工程
分子生物学分子生物学
再生医学是一种再生医学.

背景情况:

椎间盘 (IVD) 退化是由改变的微RNA (miRNA) 和Wnt信号通路的特征.
具体来说,miR-107是下调的,而Wnt3a蛋白和β-catenin在退化的IVD中是上调的.

研究的目的:

研究miR-107/Wnt3a-β-catenin信号传导在IVD退化中的作用.
评估高压氧 (HBO) 干预在这个途径上的治疗潜力.

主要方法:

在体外和体内使用退化的核细胞 (NPC) 的体外和体内研究.
分析miRNA表达,Wnt3a mRNA和蛋白质水平,β-catenin活性和下游目标.
评估HBO治疗对信号通路和组织修复标记物的影响.

主要成果:

在退化的NPC中,HBO治疗上调了miR-107和下调了Wnt3a,化LRP6和环林D1.
miR-107直接针对Wnt3amRNA的3' UTR,抑制其表达.
HBO干预降低了Wnt3a/β-catenin信号活动,β-catenin的核转位以及MMP-3和-9的分泌.
在体内,HBO在修复的软骨中降低了Wnt3a和β-catenin的表达.

结论:

在退化的NPC中,HBO通过上调miR-107表达来抑制Wnt3a/β-catenin信号传递.
在IVD退化过程中,HBO具有保护作用.
作为IVD退行症的未来治疗策略,HBO具有潜力.

关键词:

在Wnt3a/β-catenin信号传输中.退化退化退化高压氧气的高压氧气.椎间盘之间的椎间盘.这是一个微型RNA.

更多相关视频

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

A Proinflammatory, Degenerative Organ Culture Model to Simulate Early-Stage Intervertebral Disc Disease.

Published on: February 14, 2021

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits

Operational and Intervention Effects of Targeted Tuina in Lumbar Intervertebral Disc Degeneration Model Rabbits

Published on: July 21, 2023

相关概念视频

Herniated Intervertebral Disc l: Introduction

Herniated Intervertebral Disc l: Introduction

Intervertebral disc herniation refers to the displacement of the nucleus pulposus (the gel-like inner core of the disc) through a tear or weakened area in the annulus fibrosus (the outer fibrous ring). The displaced disc material extends beyond the normal boundaries of the disc space and may compress or irritate nearby spinal nerve roots or, less commonly, the spinal cord.Etiology and Risk FactorsHerniation commonly results from degeneration, in which aging reduces disc hydration and...

Degenerative Disc Disease I: Introduction

Degenerative Disc Disease I: Introduction

Degenerative disc disease is a chronic condition in which intervertebral discs gradually lose structure and function. It is not infectious or autoimmune; rather, it results from age-related biochemical and mechanical changes, influenced by genetic, metabolic, and environmental factors.Structure and Function of DiscsThe spine contains 23 intervertebral discs that absorb load, distribute forces, maintain spacing, and allow flexibility. Each disc consists of a nucleus pulposus, a gel-like core...

Degenerative Disc Disease ll: Pathophysiology

Degenerative Disc Disease ll: Pathophysiology

The symptoms of degenerative disc disease arise from a combination of mechanical compression, vascular compromise, and biochemical inflammation, which together disrupt nerve function and produce pain.Mechanical CompressionDisc degeneration reduces height and elasticity, predisposing to herniation of the nucleus pulposus, a major cause of radicular pain. Herniations may be protrusion (bulging with intact annulus), extrusion (nucleus extends beyond disc but remains connected), or sequestration...