在小鼠肝脏每日基因转录期间调节Pol II暂停
1College of Life Sciences, Wuhan University, Wuhan 430072, China.
Biology
|August 26, 2023
概括
基因表达中的昼夜节律是由转录驱动的. 这项研究揭示了RNA聚合酶II (Pol II) 暂停的全基因组变化,为每天转录节律的调节提供了新的见解.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 基因组学就是基因组学.
背景情况:
- 细胞自主昼夜振荡控制着组织间基因表达的日常节奏.
- 转录调节被认为是这些基因表达节奏的主要驱动因素.
- 转录的关键监管步骤,包括Pol II招募,暂停释放和终止,在昼夜控制的背景下仍然不完全理解.
研究的目的:
- 审查关于昼夜转录调节的当前知识,强调解释促进器-近位节律的局限性.
- 提出关于Pol II全基因组特征的新发现,在日常周期中暂停动态.
- 探索每日Pol II暂停变化的潜在调节机制及其对昼夜转录的意义.
主要方法:
- 关于昼夜转录调节的历史和最近文献的综述.
- 在24小时内对RNA聚合酶II (Pol II) 在小鼠肝脏中暂停的全基因组分析.
- 生物信息分析以表征Pol II在转录开始地点附近暂停的每日变化.
主要成果:
- 在小鼠肝脏的每日循环中,Pol II暂停表现出显著的全基因组变化.
- 在转录开始地点 (TSS) 附近的Pol II暂停中的这些动态变化对于调节转录输出至关重要.
- 这些发现表明,Pol II动态在产生节奏转录中起着至关重要的作用.
结论:
- 波尔II暂停的每日变化是昼夜转录调节的一个关键特征.
- 了解TSS的Pol II动态对于一个完整的昼夜基因表达模型至关重要.
- 这项工作为未来研究控制节奏转录的机制提供了基础.
更多相关视频
10:59Artificial RNA Polymerase II Elongation Complexes for Dissecting Co-transcriptional RNA Processing Events
Published on: May 13, 2019
9.8K
09:21Saccharomyces cerevisiae Metabolic Labeling with 4-thiouracil and the Quantification of Newly Synthesized mRNA As a Proxy for RNA Polymerase II Activity
Published on: October 22, 2018
9.2K
相关概念视频
Chromatin Structure Regulates pre-mRNA Processing
7.0K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
7.0K
Regulation of Expression at Multiple Steps
943
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
943
Cell Specific Gene Expression
13.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
13.6K
Transcription Elongation Factors
10.9K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
10.9K
Regulation of Expression Occurs at Multiple Steps
22.8K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.8K
RNA Polymerase II Accessory Proteins
9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K
