外源的斯蒙酸减轻了米中LOX3敲击所引起的爆发阻力降低
Shunyu Su1,2, Ping Tang1,2, Rubin Zuo1,2
1State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming 650201, China.
Biomolecules
|August 26, 2023
概括
在大米中删除Lipoxygenase 3 (LOX3) 基因可以提高种子质量,但可以降低对疾病的抵抗力. 贾斯蒙酸 (JA) 治疗可以恢复LOX3淘汰米的防御反应,从而为农业带来好处.
科学领域:
- 植物科学 植物科学
- 分子生物学分子生物学
- 农业科学 农业科学
背景情况:
- 胚胎中的脂氧酶3 (LOX3) 对种子质量产生影响.
- 删除LOX3基因提高了种子质量,但损害了对大米爆发和干旱的抵抗力.
研究的目的:
- 调查LOX3删除对米质量和疾病耐药性的相反影响.
- 分析斯酸 (JA) 在LOX3淘汰米中减轻爆炸易感性的作用.
主要方法:
- 产生了一个LOX3淘汰赛 (ΔLox3) 米线 (Oryza sativa L.).
- 在 ΔLox3 植物中评估了爆炸抗性,基因转录水平和反应性氧物种 (ROS).
- 评估了外源JA对受感染的 ΔLox3 植物的影响.
主要成果:
- ΔLox3植物表现出正常的表型,高甲基烯酸盐和ROS,以及与种子质量相关的KEGG通路变化.
- 受感染的 ΔLox3 植物具有严重的爆发症状,防御能力降低,并增加了ROS介导的细胞死亡.
- 治疗JA减少了爆发的严重性,阻碍了叶扩张,抑制了ROS介导的细胞死亡,并促进了ΔLox3植物的防御.
结论:
- LOX3对米生长和防御至关重要;它的淘汰会提高质量,但代价是抗病能力.
- 外源JA可以恢复LOX3淘汰米的防御机制,这表明农业应用的潜力.
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