评估氧化应激诱导的口腔上皮质毒性
Ali I Mohammed1,2, Simran Sangha1, Huynh Nguyen1
1Melbourne Dental School, The University of Melbourne, Carlton, VIC 3053, Australia.
Biomolecules
|August 26, 2023
概括
这项研究建立了一个可靠的氧化应激模型在口腔角质细胞,以了解口腔粘膜炎. 研究人员确定了研究氧化应激诱导的口腔上皮质毒性的最佳条件,这对于癌症治疗副作用至关重要.
科学领域:
- 生物化学和分子生物学
- 细胞生物学 细胞生物学
- 毒理学 毒理学 毒理学
背景情况:
- 过度的活性氧物种 (ROS) 生产导致氧化应激和细胞损伤.
- 氧化应激与口腔粘膜疾病有关,特别是口腔粘膜炎,这是癌症治疗的常见副作用.
- 口腔角质细胞对氧化应激的敏感性给开发准确的口腔疾病模型带来了挑战.
研究的目的:
- 描述与口腔毒性相关的氧化应激特征.
- 为了重现癌症治疗诱导的口腔粘膜炎的初始事件.
- 为研究氧化应激诱导的口腔上皮质毒性建立最佳的实验条件.
主要方法:
- 使用了三个口腔上皮细胞系 (OKF6,H357,H400) 和一种粘膜炎的小鼠模型.
- 将细胞暴露在不同度的过氧化 (H2O2) 中,以诱导氧化应激.
- 评估了ROS的产生,细胞活力 (trypan blue,活死试验,FCA) 和形态变化;在小鼠模型中测量了氧化应激标志物 (MPO,MDA).
主要成果:
- 在氧化应激诱导的上皮损伤期间,ROS产生的时间依赖性增加被证明.
- 观察到对口腔上皮细胞存活的显著影响,取决于氧化应激剂量和细胞系.
- 在化疗诱导的粘膜炎的小鼠模型中证实氧化应激标志物 (MPO,MDA) 的增加.
结论:
- 在人类口腔角质细胞中成功地表征和验证了氧化应激模型.
- 确定了用于研究氧化应激诱导的口腔上皮质毒性的最佳实验条件.
- 这些发现为开发和改进基于上皮细胞的口腔疾病模型提供了基础.
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