的cGAS-STING通路通过细胞亡来发挥干扰素独立的抗病毒功能
Sen Jiang1,2,3,4, Mengjia Lv1,2,3,4, Desheng Zhang1,2,3,4
1Comparative Medicine Research Institute, Yangzhou University, Yangzhou 225009, China.
Animals : an open access journal from MDPI
|August 26, 2023
概括
刺 (chSTING) 显示了独立于干扰素 (IFN) 的抗病毒活性. 这一功能涉及触发亡,而IRF7在IFN生产之外发挥着关键作用.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 细胞生物学 细胞生物学
背景情况:
- 干扰素基因 (cGAS-STING) 的循环GMP-AMP合成酶刺激通路对于先天免疫非常重要.
- 最近的发现强调了这种途径的干扰素 (IFN) 独立的抗病毒作用.
- 肉 (chSTING) 在IFN独立抗病毒反应中的特定功能仍然不清楚.
研究的目的:
- 为了调查的STING (chSTING) 是否具有独立于IFN的抗病毒活性.
- 阐明chSTING潜在的IFN独立抗病毒功能背后的机制.
主要方法:
- 在HEK293细胞和初级细胞中进行了实验.
- 检测包括评估抗病毒活性,亡和自诱导.
- 使用IRF7-淘汰的巨来检查IRF7的作用.
- 用亡和自的抑制剂来探测途径的参与.
主要成果:
- chSTING在两种细胞类型中都表现出抗病毒活性,独立于IFN生产.
- 在没有IFN的情况下,chSTING诱导了亡和自.
- 亡抑制剂,但不是自抑制剂,逆转了chSTING的抗病毒作用.
- chSTING的抗病毒功能在IRF7-淘汰的巨中被废除,这表明IRF7的参与超出了IFN信号传递.
结论:
- 刺 (chSTING) 具有独立于IFN的抗病毒功能.
- 亡似乎是调解chSTING的IFN独立抗病毒活性的一个关键机制.
- 在chSTING的抗病毒功能中,IRF7起着至关重要的作用,包括其在IFN生产中的作用之外的apoptotic效应.
相关概念视频
The Extrinsic Apoptotic Pathway
6.4K
The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.4K
The Intrinsic Apoptotic Pathway
6.6K
Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.6K
Caspases
12.6K
Caspase, a family of cysteine proteases, serve as effectors in apoptosis. The ced3 gene in C.elegans was first identified to be involved in apoptosis. This gene encodes the ced-3 caspase that is similar to the interleukin-1-beta converting enzyme or ICE in mammals. In addition to apoptosis, caspases also function in the inflammatory response. Inflammatory caspases are essential in activating pro-inflammatory cytokines that recruit immune cells and block the replication of pathogens inside...
12.6K
Immune Response Against Viral Pathogens
824
The immune system's response to viral infections is a complex and coordinated process involving natural killer (NK) cells, T cell-mediated responses, and antibody-mediated responses.
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
NK Cells
NK cells are a crucial part of our innate immune system, acting as the first line of defense against viral infections. These cells can recognize and kill infected cells without prior exposure to the virus, effectively slowing down the spread of infection. Additionally, NK cells produce proinflammatory...
824
Cells of the Innate Immune Response
1.7K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
1.7K
The JAK-STAT Signaling Pathway
9.0K
Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...
9.0K


