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牛疹病毒1的UL50 (dUTPase) 基因的分子表征

Farzana Shahin^1,2, Sohail Raza^1,2,3, Xi Chen²

¹The State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China.

Animals : an open access journal from MDPI

|August 26, 2023

概括

牛疹病毒-1 (BoHV-1) UL50蛋白质,脱氧氨酸三酸核酸酶 (dUTPase),对于病毒复制至关重要. 这项研究成功地在感染细胞中克隆,表达和检测UL50,揭示了它的细胞质局部.

科学领域:

兽医病毒学兽医病毒学
分子生物学分子生物学
生物信息学是一种生物信息学.

背景情况:

牛疹病毒-1 (BoHV-1) 在牛群中引起重大健康问题,包括呼吸道和生殖器疾病.
了解病毒蛋白的功能是控制BoHV-1感染的关键.
脱氧氨酸三酸核酸酶 (dUTPase),UL50,是BoHV-1基因产物,参与病毒复制.

研究的目的:

为了分析BoHV-1 UL50 (dUTPase) 蛋白序列和功能.
为了克隆,表达和净化BoHV-1 UL50蛋白质.
为了产生针对BoHV-1 UL50的多克隆抗血清,并确定其细胞局部.

主要方法:

对UL50氨基酸序列的生物信息分析.
在大肠杆菌中克隆和表达BoHV-1 UL50.
蛋白质净化,子的抗体产生,西部斑点和免疫光测试.

主要成果:

博赫维-1 UL50基因由978个核酸组成,编码了323个氨基酸.
再组合UL50蛋白质的分子重量约为34kDa,通过Western blot.
免疫光分析证实UL50在受感染细胞的细胞质中定位.

结论:

在疹病毒中,UL50蛋白高度保存.
在感染细胞中成功克隆,表达和检测BoHV-1 UL50.
UL50的细胞质局部化表明它在促进BoHV-1复制中的作用.

关键词:

这种病毒是BoHV-1病毒.UL50 UL50 标准的使用情况.克隆,克隆的人.在 dUTPase 中.局部化的局部化的局部化这是一种多克隆抗血清.

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