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射的短期存储:精子质量和细菌特征的差异在选定的商业扩展器中

Eva Tvrdá¹, Michaela Petrovičová², Michal Ďuračka³

¹Institute of Biotechnology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia.

Antibiotics (Basel, Switzerland)

|August 26, 2023

概括

精液扩展剂中的金素 (KAN) 改善了精子质量,减少了细菌污染. 然而,单独使用KAN并不能消除所有细菌,这凸显了优化抗生素组合和定期查在家禽精液保存方面的需求.

科学领域:

动物科学动物科学
微生物学微生物学
生殖生物学生殖生物学

背景情况:

精液的细菌污染会减少家禽授精剂的保质期.
抗生素 (ATB) 通常被添加到精液扩展剂中,以对抗细菌污染.
抗菌素耐药性增加需要评估替代或优化的抗生素策略.

研究的目的:

评估商用含有和不含甲胺 (KAN) 的精液扩展剂在预防射精的细菌污染方面的有效性.
评估KAN补充扩展剂在储存期间对精子质量参数的影响.
为了识别扩展精液样本中存在的细菌物种.

主要方法:

63只公的精液样本使用两种商业扩展剂 (无KAN和KAN补充) 和对照剂 (PBS) 进行扩展.
扩展精液在4°C下储存了24小时,评估时间为2小时和24小时.
分析了精子的运动性,活力,线粒体活动,DNA完整性和氧化特征.
使用选择性介质量化细菌负载,并通过MALDI-TOF质谱识别物种.

主要成果:

与无KAN扩展剂相比,含KAN的精液扩展剂显著改善了精子质量的保存.
扩展器与ATB补充时,细菌负载显著降低 (p ≤ 0.001).
单独的KAN不足以消灭所有细菌;仍然检测到大肠杆菌,菌菌,菌和微型菌.

结论:

含有甲胺素的精液扩展剂可以提高精子的保存率,并减少细菌污染.
在家禽精液扩展器中优化抗生素组合和剂量至关重要.
建议定期对本地和扩展的家禽精液进行细菌学查,以有效管理污染.

关键词:

马尔迪 - 托夫细菌污染细菌污染细菌的耐药性细菌的耐药性卡纳米辛是什么卡纳米辛是一种这些都是子,子,子.精液扩展器是一种精液扩展器.

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