控制尺寸的氨基基同聚合物作为广泛的抗菌剂
Meltem Haktaniyan1, Richa Sharma1, Mark Bradley1,2
1EaStCHEM, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, Edinburgh EH9 3FJ, UK.
Antibiotics (Basel, Switzerland)
|August 26, 2023
概括
合成了含有氨基团的抗微生物聚合物,并对细菌进行了测试. 最有效的聚合物,聚二甲基化,通过破坏细菌膜,显示出广泛的抗菌活性.
科学领域:
- 聚合物化学 聚合物化学
- 抗菌剂 抗菌剂 抗菌剂
- 生物材料是一种生物材料.
背景情况:
- 有基的聚合物表现出天然的抗微生物特性.
- 这些聚合物可以预防或消除微生物引起的感染.
- 开发新的抗菌剂对于打击微生物耐药性至关重要.
研究的目的:
- 合成和评估含氨同聚合物作为抗菌剂.
- 为了研究分子质量对聚合物抗菌活性和毒性的影响.
- 阐明这些聚合物对细菌的作用机制.
主要方法:
- 同聚合物通过可逆添加碎片化链转移聚合 (RAFT) 来合成.
- 对抗微生物活性进行了测试,对抗了阳性细菌 (M. luteus, B. subtilis) 和阴性细菌 (E. coli, S. typhimurium).
- 使用基于染料的测试和扫描电子显微镜 (SEM) 研究了作用机制.
主要成果:
- 三种聚合物被合成并以不同的分子量 (10,20,40kDa) 进行测试.
- 聚二甲基基化物) (40 kDa) 显示出最高的非格兰特异性活性 (MIC 16-64 μg/mL).
- SEM揭示了细菌细胞外的破坏;最活跃的聚合物通过向细胞质膜而具有杀菌作用.
结论:
- 聚二甲基化 (Poly ((2-(methacryloyloxy) ethyl]trimethylammonium chloride) 是一种强效的非血解性抗菌剂.
- 这种聚合物的有效性与其分子量和破坏细菌膜的能力有关.
- 潜在的生物医学应用包括表面和治疗眼睛感染.
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