活跃和康复的COVID-19患者的乳盘特征使用下一代蛋白质组学技术揭示,SWATH-MS
Iván Hernández-Caravaca1,2, Carla Moros-Nicolás2,3, Leopoldo González-Brusi2,3
1Department of Community Nursing, Preventive Medicine and Public Health and History of Science, Campus de Sant Vicent del Raspeig, University of Alicante, 03690 Alicante, Spain.
Children (Basel, Switzerland)
|August 26, 2023
概括
人类胆乳液不会将SARS-CoV-2 (导致COVID-19的病毒) 从母亲传播给婴儿. 分析揭示了胆乳中特定的蛋白质配置文件,这可能为新生儿提供免疫益处.
科学领域:
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 大奶粉为新生儿提供必要的营养,免疫和发育益处.
- 有关严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 通过母乳传播存在担忧.
- 有限的证据表明,母乳对抗SARS-CoV-2感染的保护作用有限.
研究的目的:
- 为了分析人体胆汁中SARS-CoV-2的存在.
- 为了识别活跃COVID-19的母亲和康复的患者的乳酪蛋白的蛋白质表达特征.
- 评估乳母乳对婴儿的潜在免疫益处.
主要方法:
- 从三个组收集的结肠杆菌样本:活跃的COVID-19,康复的COVID-19,和未感染的妇女.
- 用RT-qPCR检测SARS-CoV-2RNA. 在检测SARS-CoV-2RNA时使用.
- 进行SWATH-MS和生物信息分析,用于蛋白质基因分析.
主要成果:
- 在COVID-19患者的胆乳样本中没有检测到SARS-CoV-2.
- 蛋白质组分析在所有样本中确定了301种常见的蛋白质.
- 11种蛋白质被确定为活跃COVID-19感染的潜在生物标志物,主要与免疫和炎症有关.
结论:
- 人类胆乳不太可能成为母婴SARS-CoV-2传播的途径.
- 活跃或已康复COVID-19的母亲的乳母蛋白可能会给婴儿带来免疫优势.
- 进一步研究胆乳的免疫调节特性是有必要的.
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