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TRIM25通过激活RIG-1-介导型I干扰子来抑制狂犬病病毒固定HEP-Flury菌株的产生

Boyue Zhang¹, Ting Cai¹, Hongling He¹

¹College of Veterinary Medicine, South China Agricultural University, Guangzhou 510651, China.

|August 26, 2023

概括

含有25 (TRIM25) 的三方基因作为对狂犬病病毒 (RABV) 的宿主限制因子. 提升TRIM25的调节通过激活可诱导视网膜酸基因I (RIG-I) 和I型干扰素通路来抑制RABV的复制.

科学领域:

病毒学病毒学
免疫学免疫学免疫学
分子生物学分子生物学

背景情况:

狂犬病病毒 (RABV) 构成全球重大公共卫生风险,感染机制尚不完全理解,治疗选择有限.
宿主限制因素对于控制病毒复制和开发抗病毒疗法至关重要.
含三方基因 (TRIM) 蛋白质是已知的病毒复制和先天免疫反应的调节者.

研究的目的:

调查TRIM25在RABV感染中的作用.
阐明TRIM25影响RABV复制的机制.
确定TRIM25作为狂犬病的潜在治疗点.

主要方法:

下一代测序用于评估感染期间的TRIM25表达.
基因淘汰和过度表达技术用于研究TRIM25功能.
干扰素和RIG-I敲击实验分析了所涉及的信号通路.
对TRIM25与病毒蛋白相互作用的分析.

主要成果:

在HEP-Flury感染期间,TRIM25的表达上调.
TRIM25 Knockdown 增强了病毒的产生,而过度表达则抑制了病毒的产生.
TRIM25通过准RIG-I来调节I型干扰子反应,这表明RIG-I-IFN轴的参与.
TRIM25与HEP-Flury结构蛋白没有直接相互作用.

结论:

TRIM25作为对抗RABV感染的宿主限制因子.
TRIM25通过RIG-I-干扰素信号通路抑制RABV复制.
TRIM25代表了针对狂犬病的抗病毒药物开发的潜在新目标.

关键词:

在RIG-I中,RIG-I是指RIG-I.在 TRIM2525 中,干扰子干扰子干扰子狂犬病病毒病毒的病毒.无处不在的化

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