血液转录基因分析揭示了与孕母羊暴露在环境热应激中的耐热性相关的功能途径
Rosa I Luna-Ramirez1, Sean W Limesand1, Ravi Goyal1
1School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, AZ 85721, USA.
Genes
|August 26, 2023
概括
绵羊具有耐热压力耐受性的遗传机制. 这项研究确定了关键的基因和免疫通路,如亡,参与调节母羊的耐热性,为它们的分子基础提供了洞察力.
科学领域:
- 动物基因组学 动物基因组学
- 环境生理学环境生理学
- 分子生物学分子生物学
背景情况:
- 环境热应激对畜牧业生产构成重大挑战.
- 绵羊表现出不同程度的耐热性,受遗传因素的影响.
- 了解耐热性分子基础对于改善动物福利和生产力至关重要.
研究的目的:
- 鉴定暴露于热应激的母羊中与耐热性相关的基因和调节途径.
- 研究遗传调节在羊对环境热量反应中的作用.
- 阐明在羊群中耐热应激的分子机制.
主要方法:
- 下一代RNA测序是在分类为耐受性或非耐受性的热应激母羊的血液样本上进行的.
- 不同基因表达分析确定了358个显著基因.
- 进行了基因本体学 (GO) 和KEGG通路分析,并对高度调节的基因进行了网络分析.
主要成果:
- 鉴定了358个差异表达的基因,p53,RIG-I类受体和FoxO信号通路的显著丰富.
- 具有高调节潜力的关键过度表达基因包括ATM,ISG15,IRF7,MDM4,DHX58和TGFβR1.
- 在淋巴细胞和单细胞中观察到免疫相关基因 (ISG15,IRF7,DHX58) 的共同表达网络,与血液学发现相关.
结论:
- 转录基因分析揭示了一种非病毒性免疫机制,涉及亡,作为雌羊耐热应激耐受力的关键组成部分.
- 这些发现凸显了亡和免疫反应途径在热耐性分子调节中的重要性.
- 鉴定的基因和途径提供了通过遗传策略增强绵羊热应激抵抗力的潜在目标.
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