3'非编码序列负面调节PD-L1表达,其调节者在泛癌中被系统地识别出来
Zike Chen1, Hui Pi2, Wen Zheng3
1Laboratory of Medical Science, School of Medicine, Nantong University, Nantong 226001, China.
Genes
|August 26, 2023
概括
在PD-L1的3'-未翻译区域 (3'-UTR) 显著调节PD-L1水平. 删除3'-UTR可以调节PD-L1,而已识别的调节者可以预测免疫疗法反应.
科学领域:
- 分子生物学分子生物学
- 癌症研究 癌症研究
- 免疫学 免疫学 免疫学
背景情况:
- 尽管PD-L1的3-非翻译区域 (3-UTR) 很长,但其大部分未被研究.
- 了解PD-L1调节对于癌症免疫治疗至关重要.
研究的目的:
- 研究PD-L1 3 -UTR在调节PD-L1表达中的作用.
- 确定PD-L1的新型调节剂及其作为免疫治疗反应生物标志物的潜力.
主要方法:
- 在CRISPR-Cas9基因编辑中删除PD-L1 3 -UTR.
- 生物信息分析包括预后,免疫透和microRNA网络分析.
- 对具有PD-L1表达的RNA结合蛋白 (RBPs),microRNA和m6A调节者的相关性分析.
主要成果:
- 删除PD-L1 3 -UTR显著上调的PD-L1表达.
- 确定了43个RBP,38个microRNA和21个与PD-L1.1相关的m6A调节器.
- 开发了一种新的免疫特征 (RBMS1,QKI,ZC3HAV1,RBM38) 用于预测免疫治疗反应.
结论:
- 在PD-L1调节中,PD-L1 3 -UTR起着至关重要的作用.
- 已识别的调节剂和开发的签名显示了作为癌症预后和预测免疫疗法疗效的生物标志物的潜力.
更多相关视频
10:11Immunohistochemical Staining of B7-H1 PD-L1 on Paraffin-embedded Slides of Pancreatic Adenocarcinoma Tissue
Published on: January 3, 2013
23.2K
10:29Semi-automatic PD-L1 Characterization and Enumeration of Circulating Tumor Cells from Non-small Cell Lung Cancer Patients by Immunofluorescence
Published on: August 14, 2019
10.6K
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.6K
02:23Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K