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对于D-氨酸生产的表皮酶的工程,表达和固定

Jin Hao Tan¹, Anqi Chen², Jiawu Bi^3,4

¹Microbial Cell Bioprocessing, Bioprocessing Technology Institute, Agency for Science, Technology and Research (A*STAR), Singapore 138668, Singapore.

International journal of molecular sciences

|August 26, 2023

概括

研究人员已经确定了新的酶和方法来改善罕见的糖D-allulose的生产. 这些进步提高了酶稳定性和转化率,为工业规模的D-粉制造铺平了道路.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
碳水化合物化学碳水化合物化学

背景情况:

一种罕见的糖,D-糖,作为糖的替代品,为健康提供了好处.
目前使用D-氨酸表皮酶 (DAEase) 酶的生产方法面临着耐热性差和低转化率等局限性.
现有的酶包括DAEase,D-psicose-3-epimerase (DPEase) 和D-tagatose-3-epimerase (DTEase) 等.

研究的目的:

审查D-粉生产的最新进展.
突出新发现的适用于工业应用的DAEases.
讨论提高D-粉制造效率和可扩展性的策略.

主要方法:

鉴定和描述新型DAEases.
酶工程是为了提高耐热性和抗酸性.
生物技术方法,包括使用Bacillus subtilis进行生物合成.
酶固定化技术以增强活性,稳定性和半衰期.

主要成果:

发现了三种新的DAEases,其特性有利于工业D-粉生产.
成功设计了DAEases,以提高稳定性和耐力.
通过各种增强策略,通过各种增强策略,证明了D-氨酸产量的改善.
在弥合实验室规模和工业规模D-粉制造之间的差距方面取得了进展.

结论:

最近的研究为高效的工业规模D-粉生产提供了有前途的解决方案.
新型酶和先进技术显著提高了产量和工艺可行性.
这些进展支持D-粉素作为一种更健康的糖替代品的更广泛的可用性.

关键词:

这种细菌是 Bacillus subtilis.D - 氨酸 D-氨酸 D-氨酸埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.生物制造生物制造生物制造金属纳米粒子是一种金属纳米粒子.蛋白质工程工程蛋白质工程稀有糖是一种罕见的糖.甜味剂甜味剂甜味剂甜味剂

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