慢性压力改变了肠道透性和诱导炎症,被雌激素恢复
Yuanyuan Li1, Huayun Wan1, Ruiqin Ma1
1Department of Basic Veterinary Medicine, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China.
慢性压力会伤害怀孕的小鼠.
科学领域:
- 身体生理学 身体生理学
- 微生物组研究 微生物组研究
- 压力内分泌学 内分泌学
背景情况:
- 慢性心理压力会对健康产生负面影响,尤其是孕妇.
- 压力对孕动物肠道屏障和肠道微生物群的影响尚未完全理解.
研究的目的:
- 为了研究慢性压力对肠道粘膜屏障和结肠微生物组合在怀孕小鼠的影响.
- 探索雌二醇 (E2) 在减轻压力引起的肠损伤中的保护作用.
主要方法:
- 创建了一个压力诱导的模型,使用八周大的怀孕小鼠进行克制压力.
- 测量了上腺素 (NE),皮质 (CORT) 和雌激素 (E2) 的血水平.
- 结肠组织分析了炎症性细胞因子,细胞增殖,亡标记物 (caspase-3) 和紧结的表达.
- 评估了结肠微生物群的多样性,丰富性和组成.
- 在体外实验中评估了E2对肠道上皮细胞对过氧化 (H2O2) 损伤的保护作用.
主要成果:
- 压力显著增加了血NE,CORT和E2水平.
- 压力导致结肠粘膜损伤,其特征是杯状细胞减少,细胞核抗原 (PCNA) 阳性细胞增殖,以及紧密结节表达.
- 促炎性细胞因子被上调,而抗炎性细胞因子被下调.
- 结肠微生物群的多样性和丰富性下降,细菌群减少,潜在有害细菌增加.
- 雌激醇 (E2) 证明对肠道上皮细胞具有保护作用,25 pg/mL E2 显示最佳保护.
结论:
- 慢性压力显著损害了肠道粘膜屏障,并改变了怀孕小鼠的肠道微生物群组成.
- 雌激醇 (E2) 在压力条件下在维持肠道屏障完整性方面起着保护作用.
- 准E2可能提供一种治疗策略,用于在怀孕期间管理压力诱导的胃肠道问题.
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