相关实验视频

Updated: Jul 18, 2025

Operating Procedures of the Electrochemotherapy for Treatment of Tumor in Dogs and Cats

Operating Procedures of the Electrochemotherapy for Treatment of Tumor in Dogs and Cats

Published on: October 24, 2016

用IL-2和IL-12进行结肠癌基因免疫疗法,使用基因电转移

Tilen Komel^1,2, Masa Omerzel¹, Urska Kamensek¹

¹Department of Experimental Oncology, Institute of Oncology Ljubljana, Zaloska 2, SI-1000 Ljubljana, Slovenia.

International journal of molecular sciences

|August 26, 2023

概括

使用介素2和介素12基因电转移的基因免疫疗法在小鼠中消除了结肠瘤. 这种组合疗法刺激了抗瘤免疫力,并创造了持久的免疫记忆.

科学领域:

免疫治疗是一种免疫疗法.
在瘤学瘤学.
分子生物学分子生物学

背景情况:

基因免疫疗法是癌症治疗的一个关键策略.
介素2 (IL-2) 和介素12 (IL-12) 基因刺激抗瘤免疫反应.

研究的目的:

为了评估IL-2和IL-12等离子体基因电转移在CT26小鼠结肠癌中的疗效.
评估IL-2和IL-12基因治疗的综合作用.

主要方法:

在实验室优化基因电转移脉冲协议用于IL-2和IL-12表达.
在小鼠中使用CT26结肠癌模型的体内研究.
对瘤生长,免疫细胞透和细胞因子水平的分析.

主要成果:

结合IL-2和IL-12基因电转移显著延迟了瘤的生长,并诱导了小鼠的完整反应.
与对照组相比,组合疗法导致免疫刺激性细胞因子的升高和免疫细胞透的增加.
建立了长期的抗瘤免疫力,通过成功的瘤再挑战来证明.

结论:

在小鼠模型中,IL-2和IL-12的组合基因电转移是消除CT26结肠癌的有效策略.
这种方法产生了强大的抗瘤免疫记忆,表明潜在的持久临床益处.

关键词:

CT26结肠癌结肠癌在IL-12中,IL-12是最重要的.在IL-2中,IL-2被认为是IL-2.抗瘤免疫记忆抗瘤免疫记忆基因电转移是一种基因电转移.

更多相关视频

Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

Ex vivo Expansion of Tumor-reactive T Cells by Means of Bryostatin 1/Ionomycin and the Common Gamma Chain Cytokines Formulation

Published on: January 14, 2011

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Experimental Melanoma Immunotherapy Model Using Tumor Vaccination with a Hematopoietic Cytokine

Published on: February 24, 2023

相关概念视频

Tumor Immunotherapy

Tumor Immunotherapy

Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.

Gene Therapy

Gene Therapy

Gene therapy is a technique where a gene is inserted into a person’s cells to prevent or treat a serious disease. The added gene may be a healthy version of the gene that is mutated in the patient, or it could be a different gene that inactivates or compensates for the patient’s disease-causing gene. For example, in patients with severe combined immunodeficiency (SCID) due to a mutation in the gene for the enzyme adenosine deaminase, a functioning version of the gene can be...