进行比较的转录基因组分析,发现两种 WRKY 转录因子在 Polygonatum cyrtonema 中积极调节多糖生物合成
Wu Jiang1,2, Jiadong Chen1, Xiaojing Duan1
1Zhejiang Institute of Subtropical Crops, Zhejiang Academy of Agricultural Sciences, Wenzhou 325005, China.
International journal of molecular sciences
|August 26, 2023
概括
阐明了Polygonatum cyrtonema多糖体 (PCP) 的生物合成过程. 确定了诸如UDP-glucose 4-epimerase (galE) 和转录因子PcWRKY31/34之类的关键酶,使这种药用草本能够进行分子繁殖.
科学领域:
- 植物生物化学 植物生物化学
- 分子生物学分子生物学
- 传统中国医药 传统中国医药
背景情况:
- 波利格纳 (Polygonatum cyrtonema,P. cyrtonema) 是一种重要的药用草本.
- 多糖 (PCP) 是其主要的生物活性化合物.
- 目前尚不清楚PCP生物合成的分子机制.
研究的目的:
- 研究P. cyrtonema中PCP生物合成的分子基础.
- 确定PCP生产中关键的酶和调控因素.
- 为分子辅助繁殖P.cyrtonema提供见解.
主要方法:
- 在不同P.cyrtonema生殖质中PCP的量化.
- 高和低PCP的转录组测序产生生殖质.
- 定量实时PCR (qRT-PCR) 用于分析差异性基因表达.
- 基因克隆,蛋白质局部化,短暂的过度表达,光酶试验和酵母单杂交 (Y1H) 试验.
主要成果:
- 在胚胎等离子体中观察到PCP含量的显著变化.
- 鉴定出UDP-葡萄糖4-表皮酶 (galE) 和GDP-曼诺4,6-脱水酶是关键酶.
- galE1蛋白定位在叶绿体上,其过度表达增加了PCP含量.
- 发现PcWRKY31和PcWRKY34转录因子可以激活galE1的表达.
结论:
- 发现了P.cyrtonema中PCP生物合成的新型调节机制,其中涉及galE1和PcWRKY转录因子.
- 这项研究为了解PCP生物合成和开发P. cyrtonema的分子育种策略提供了基础.
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