相关实验视频

Updated: Jul 18, 2025

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Rapid One-step Enzymatic Synthesis and All-aqueous Purification of Trehalose Analogues

Published on: February 17, 2017

在回收批次模式下在三糖银化中应用生理聚合的LaCZ

Martina Belkova^1,2, Tatiana Janegova^1,2, Eva Hrabarova^1,2

¹Institute of Chemistry, Centre for Glycomics, Slovak Academy of Sciences, Dubravska Cesta 9, SK-84538 Bratislava, Slovakia.

Life (Basel, Switzerland)

|August 26, 2023

概括

基于三糖的新方法制造银河脂糖 (GOS) 使用固定化的酶. β-galactosidase的活性纳入体 (aIB) 为食品和制药应用中高效的GOS生产提供了一种新的方法.

科学领域:

生物技术是生物技术.
酶工程是什么? 酶工程是什么?
碳水化合物化学碳水化合物化学

背景情况:

галактоолигосахарид (GOS) 是具有健康益处的公认益生菌.
基于三糖的GOS为食品和制药行业提供了机会.
为了高效的GOS生产,需要新的酶固定化技术.

研究的目的:

开发和评估生产基于三糖的新型方法,以制造基于三糖的银河酸.
通过使用活性纳入体 (aIBs) 进行三糖银化来研究固定β-银酸酶的疗效.

主要方法:

再组合的LacZβ-galactosidase被用一种易聚合的标签设计,以形成活性纳入体 (aIB).
aIBs 在回收批量模式下用于三糖银河系酶化.
测试了各种固定化策略,包括酸盐珠,酸盐/纤维素硫酸盐/聚甲-协同瓜尼丁 (PMCG) 囊和磁性aIBs.

主要成果:

自由的aIBs和酸盐被困的aIBs与封装在酸盐/纤维素硫酸盐/PMCG囊中的aIBs进行了比较.
酸盐/纤维素硫酸盐/PMCG囊显示出优越的生物转化性能.
优化的系统在高度 (25% w/v) 和高温 (50 °C) 时有效地转化了三糖.

结论:

活性纳入体 (aIB) 提供了一种有效的 in vivo 酶固定化的方法.
酸盐/纤维素酸盐/PMCG封装增强了aIBs在工业生物转化中的稳定性和适用性.
这种方法提供了一个有前途的策略,用于可扩展的生产基于三糖的银酸.

关键词:

在 LacZ LacZ 的位置上积极包容性机构是积极包容性机构.阿尔金酸盐珠子珠子封装的封装方式银河系氧糖酸盐 (galactooligosaccharides) 是一种酸盐.在活体中,酶固定化.磁化是一种磁化.三糖的银河系氧化.这就是β-galactosidase.

更多相关视频

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Published on: February 5, 2018

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Analysis of Fucosylated Human Milk Trisaccharides in Biotechnological Context Using Genetically Encoded Biosensors

Published on: April 13, 2019

相关概念视频

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Protein Folding Quality Check in the RER

Protein Folding Quality Check in the RER

ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...