通过有机酸的阴离子衍生增强LC-ESI-MS/MS灵敏度
Tamar Shamai Yamin1, Moran Madmon1, Ariel Hindi1
1Department of Analytical Chemistry, Israel Institute for Biological Research (IIBR), P.O. Box 19, Ness Ziona 7410001, Israel.
Molecules (Basel, Switzerland)
|August 26, 2023
概括
使用CAX-B的化学衍生物显著提高了有机 (OP) 酸的检测. 这种方法可将OP环境退化剂的识别极限提高两倍.
科学领域:
- 环境化学环境化学
- 分析化学 分析化学
- 质谱测量质量谱测量
背景情况:
- 有机酸 (OP) 是OP神经毒剂和农药的关键环境降解剂.
- 准确检测这些化合物对于环境监测和安全至关重要.
- 现有的分析方法可能缺乏足够的灵敏度来检测微量水平的OP酸.
研究的目的:
- 开发和说明一种化学衍生方法,以提高OP酸的信号强度和信号噪声比.
- 通过液态染色学双重质谱法 (LC-ESI-MS/MS) 提高OP酸的鉴定极限 (LOI).
- 研究这种衍生在各种矩阵中的选和识别目标和未知的OP酸的实用性.
主要方法:
- 使用的N-(2-(甲基) 基) -N,N-二甲基乙胺酸 (CAX-B) 用于衍生.
- 分析了一组由八种OP酸组成的小组,包括基甲基酸和基酸相似物.
- 在70°C进行1小时的碳酸与乙二的衍生物化.
- 在MRM模式下使用LC-Orbitrap-ESI-MS/MS和LC-MS ((QqQ) 进行分析.
主要成果:
- 使用CAX-B的衍生品将LOI增加了一到两个以上的数量级 (从1-10 ng/mL到0.02-0.2 ng/mL).
- 在各种样本矩阵中观察到增强的灵敏度,包括头发,混凝土和植物提取物.
- 衍生酸的ESI-MS/MS光谱显示了特有的中性损失和核心有机离子,有助于识别.
结论:
- 该CAX-B衍生方法在OP酸的检测灵敏度上提供了显著的改进.
- 这种方法在复杂的环境样本中对已知和未知的OP酸进行选和识别是有效的.
- 改进的LOI使得这种方法对OP酸污染物的微量分析具有价值.
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