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转基因微藻表达双链RNA作为潜在的料补充剂,用于控制养殖水产养殖中的白斑综合征

Patai Charoonnart^1,2, Henry Nicholas Taunt³, Luyao Yang³

¹Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

|August 26, 2023

概括

研究人员开发了一种新方法,在微藻中产生抗病毒双链RNA (dsRNA),显著增加dsRNA产量. 这种增强的dsRNA有效地保护免受白斑综合征病毒 (WSSV) 感染,当它们被纳入料时.

科学领域:

水产养殖是水产养殖的一种方式.
分子生物学分子生物学
生物技术是生物技术.

背景情况:

病毒感染对养殖鱼类和贝类构成重大威胁.
使用双链RNA (dsRNA) 的RNA干扰 (RNAi) 是一个有前途的抗病毒策略.
正在探索dSRNA的口服供应用于水产养殖疾病控制.

研究的目的:

为了改善微藻中抗病毒dsrna的产生,Chlamydomonas reinhardtii.
评估增强的dsRNA在保护鱼免受白斑综合征病毒 (WSSV) 的有效性.

主要方法:

经过工程 *Chlamydomonas reinhardtii* 来表达 WSSV 特定的 dsRNA 使用一种新的促进器策略.
使用定量RT-PCR量化dSRNA的量化生产.
在WSSV挑战之前向幼虫食dsrna生成的微藻.

主要成果:

与以前的方法相比,达到了大约10倍的dsRNA产量 (~119 ng/L).
用dSRNA补充的藻类显著增强了对WSSV的生存率 (~69%).
展示了一种低成本,低技术的抗病毒dRNA生产方法.

结论:

克拉米多马纳斯强硬菌的新型dSRNA合成策略显著提高了抗病毒dSRNA产量.
这种增强的dsRNA生产平台为控制水产养殖中的WSSV提供了可行的解决方案.
这些发现支持使用工程微藻作为水产养殖疾病管理的可持续方法.

关键词:

克拉米多马纳斯病毒重新硬化这是一种RNA干扰.塑技术工程塑工程这是一种双链RNA.养殖水产养殖的.白色斑点综合征病毒病毒.

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