候选植物辐射中的金属β-乳酸酶类编码基因:广泛且高度分离的蛋白质,具有潜在的多功能性
Mohamad Maatouk1,2, Vicky Merhej1,2, Pierre Pontarotti1,2,3
1Microbes, Evolution, Phylogénie et Infection (MEPHI), Institut de Recherche pour le Développement (IRD), Assistance Publique-Hôpitaux de Marseille (AP-HM), Aix-Marseille University, 13005 Marseille, France.
Microorganisms
|August 26, 2023
概括
候选物质辐射 (CPR) 含有众多抗生素耐药性基因,包括许多具有多样性和不具特征功能的金属β-乳糖酶类 (MBL类) 折叠. 需要进一步的研究来了解它们的起源和作用.
科学领域:
- 微生物学和基因组学 微生物学和基因组学
- 酶学和蛋白质科学 酶学和蛋白质科学
背景情况:
- 候选细胞辐射 (CPR) 是一种多样化的细菌群体,其基因组在很大程度上没有特征.
- 抗生素耐药性是一个日益严重的全球健康问题,需要发现新的耐药性机制.
研究的目的:
- 在CPR基因组中调查金属β-乳糖酶样 (MBL样) 编码基因的谱.
- 执行CPRMBL样蛋白的in silico功能表征,并将其与已知的MBL进行比较.
主要方法:
- 在CPR基因组中进行MBL类折叠的in silico预测和分析.
- 与MBL数据库 (BLDB) 和表达的MBL相对应的蛋白质域配置文件的比较分析.
- 序列相似性网络 (SSN) 分析以预测CPRMBL类序列的功能集群.
主要成果:
- 鉴定了3349个CPR基因,预计具有MBL类折叠,比细菌MBL长度和多样性更大.
- 类似CPR MBL的序列具有很高的功能域含量,大多数缺乏与已知表达的MBL的连接.
- 表明在CPRMBL类蛋白超级家族中存在许多新型,未经表征的功能.
结论:
- CPR拥有庞大且高度可变的MBL类折叠谱,超过已知的MBL多样性.
- 这项研究强调了CPR中新型抗生素耐药机制的重大潜力.
- 进一步的实验和进化研究对于阐明这些酶的功能注释,起源和适应性作用至关重要.
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